Pressure-clamp PIEZO1
| . | 2-D dwell time distribution: Chronological vs. time-reversed . | Markov modeling: Log Likelihood values (fitting with M21) . | |
|---|---|---|---|
| Pipette pressure (mmHg) | χ2 test Z values | With detailed balance | Without detailed balance |
| 0 | 1.93499 | 3,529 | 3,529 |
| −20 | −2.65205 | 15,932 | 15,932 |
| −30 | −1.1922 | 36,186 | 36,186 |
| −40 | −0.55871 | 57,261 | 57,261 |
| −50 | −1.17943 | 119,000 | 119,000 |
| −65 | −1.60886 | 95,538 | 95,538 |
| −80 | −1.01035 | 119,500 | 119,500 |
| −90 | −1.44066 | 34,891 | 34,891 |
| . | 2-D dwell time distribution: Chronological vs. time-reversed . | Markov modeling: Log Likelihood values (fitting with M21) . | |
|---|---|---|---|
| Pipette pressure (mmHg) | χ2 test Z values | With detailed balance | Without detailed balance |
| 0 | 1.93499 | 3,529 | 3,529 |
| −20 | −2.65205 | 15,932 | 15,932 |
| −30 | −1.1922 | 36,186 | 36,186 |
| −40 | −0.55871 | 57,261 | 57,261 |
| −50 | −1.17943 | 119,000 | 119,000 |
| −65 | −1.60886 | 95,538 | 95,538 |
| −80 | −1.01035 | 119,500 | 119,500 |
| −90 | −1.44066 | 34,891 | 34,891 |
Single channel activity does not violate microscopic reversibility at all tested pressures.