Table 1. Characteristic values for... | Rockefeller University Press

Table 1.

Characteristic values for steady-state isometric stress and three-parameter Hill fits to the Ca²⁺-activated stress–pCa relationships

	WT		cMyBP-C KO
	AP	MLCK	AP	MLCK
F_min (kN m⁻²)	1.15 ± 0.10	1.37 ± 0.13	1.46 ± 0.12	1.55 ± 0.12
F_abs (kN m⁻²)	13.55 ± 0.55^{^a}	17.76 ± 0.85^{^b}	12.21 ± 0.51	12.49 ± 0.67
F_dev (kN m⁻²)	12.55 ± 0.53^{^a}	16.56 ± 0.85^{^b}	10.99 ± 0.47	11.15 ± 0.62
pCa₅₀	5.80 ± 0.02^{^a}	5.95 ± 0.03^{^b}	5.86 ± 0.02^{^a}	6.15 ± 0.03
n_H	6.32 ± 0.42	6.34 ± 0.68^{^b}	4.50 ± 0.38	4.48 ± 0.34
F_max (kN m⁻²)	13.06 ± 0.60^{^a}	16.50 ± 0.81^{^b}	11.14 ± 0.51	11.40 ± 0.67
n strips	21	19	18	22
n hearts	5		5

	WT		cMyBP-C KO
	AP	MLCK	AP	MLCK
F_min (kN m⁻²)	1.15 ± 0.10	1.37 ± 0.13	1.46 ± 0.12	1.55 ± 0.12
F_abs (kN m⁻²)	13.55 ± 0.55^{^a}	17.76 ± 0.85^{^b}	12.21 ± 0.51	12.49 ± 0.67
F_dev (kN m⁻²)	12.55 ± 0.53^{^a}	16.56 ± 0.85^{^b}	10.99 ± 0.47	11.15 ± 0.62
pCa₅₀	5.80 ± 0.02^{^a}	5.95 ± 0.03^{^b}	5.86 ± 0.02^{^a}	6.15 ± 0.03
n_H	6.32 ± 0.42	6.34 ± 0.68^{^b}	4.50 ± 0.38	4.48 ± 0.34
F_max (kN m⁻²)	13.06 ± 0.60^{^a}	16.50 ± 0.81^{^b}	11.14 ± 0.51	11.40 ± 0.67
n strips	21	19	18	22
n hearts	5		5

F_min, absolute stress value at pCa 8.0; F_abs, absolute stress value at pCa 4.8; F_dev, Ca²⁺-activated, developed stress (T_max − T_min); pCa₅₀, n_H, and F_max (= maximal stress) represent fit parameters to a three-parameter Hill equation for the F_dev-pCa relationship; n strips, total number of technical replicates (4–7 per heart); n hearts, number of biological replicates.

P < 0.05 effect of MLCK treatment within the same genotype.

P < 0.05 effect of genotype within same treatment type.

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