Table 2.

Primers used in this study

PrimersSequences (5′-3′)
GFP-Tex2-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​ACA​AGT​CTG​TAT​GGT​CGC​CAT​G-3′ 
GFP-Tex2-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TCA​TGG​CTG​ATC​AGC​AGC​CT-3′ 
GFP-Tex2(1-474)-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​CTA​CTT​CAC​TGG​CAA​TTC​CGG​G-3′ 
GFP-Tex2(1-540)-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TCA​GAT​ATC​CAG​AGA​TCT​TGT​GTT​CC-3′ 
GFP-Tex2(474-1131)-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​ACG​TTG​GGC​TTC​TTT​ATA​ATG​TGT​G-3′ 
GFP-Tex2(1-276)-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​CTA​GGA​ACG​AGT​ATC​AGA​GGG​AGA​A-3′ 
GFP-Tex2(277-517)-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​CGT​TCC​TTT​TTT​AAA​GTG​CCC​G-3′ 
GFP-Tex2(277-517)-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TTA​TGT​AAA​AAA​CCA​AAT​CAC​GCA​A-3′ 
GFP--Tex2(∆ 277-473)-F 5′-GTT​CCT​TTA​CGT​TGG​GCT​TCT​TTA​TAA​TGT​GTG-3′ 
GFP--Tex2(∆ 277-473)-R 5′-GCC​CAA​CGT​AAA​GGA​ACG​AGT​ATC​AGA​GGG​AGA-3′ 
Halo-Tex2-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ACA​AGT​CTG​TAT​GGT​CGC​CAT​G-3′ 
Halo-Tex2-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TCA​TGG​CTG​ATC​AGC​AGC​CT-3′ 
Tex2(TM)-GFP-F 5′-TGA​ACC​GTC​AGA​TCC​GCT​AGC​ACG​TTG​GGC​TTC​TTT​ATA​ATG​TGT​G-3′ 
Tex2(TM)-GFP-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TGT​AAA​AAA​CCA​AAT​CAC​GCA​AA-3′ 
GFP-Tex2(∆ TM)-F 5′-CAG​TGA​AGC​CAC​CAA​GTG​CTC​ATA​AAT​ATC​ACA​AG-3′ 
GFP-Tex2(∆ TM)-R 5′-ACT​TGG​TGG​CTT​CAC​TGG​CAA​TTC​CGG​GTG​CT-3′ 
Halo-Tex2(∆ PH)-F 5′-GAA​GAA​ATC​AAG​AAG​TCA​TCG​GGT​GTC​TCT​GG-3′ 
Halo-Tex2(∆ PH)-R 5′-GAC​TTC​TTG​ATT​TCT​TCT​TTG​ATA​TCC​AGA​GAT​CTT​GTG​TT-3′ 
GFP-Tex2(SMP)-F 5′-CCA​CCC​TCT​ATA​ATG​CAC​TCA​GCC​ATG​GAC​CC-3′ 
GFP-Tex2(SMP)-R 5′-TGC​ATT​ATA​GAG​GGT​GGA​ACC​TCT​GGC​AAC​TT-3′ 
GFP/Halo-Tex2(∆SMP)-F 5′-CCA​CCC​TCt​ATA​ATG​CAC​TCA​GCC​ATG​GAC​CC-3′ 
GFP/Halo-Tex2(∆SMP)-R 5′-TGC​ATT​ATa​GAG​GGT​GGA​ACC​TCT​GGC​AAC​TT-3′ 
GFP-Tex2(∆ TM-∆PH)-F 5′-CAG​TGA​AGG​AGG​AGG​AAG​AAC​AGG​AAG​CCT​GG-3′ 
GFP-Tex2(∆ TM-∆PH)-R 5′-TTC​CTC​CTC​CTT​CAC​TGG​CAA​TTC​CGG​GTG​CT-3′ 
14xHis-NEDD8-Tex2(PH)-F 5′-GGC​GCC​GGT​ACC​TGC​GGA​TCC​CCA​CCA​AGT​GCT​CAT​AAA​TAT​CAC​A-3′ 
14xHis-NEDD8-Tex2(PH)-R 5′-GCT​CAG​CTA​ATT​AAG​AAG​CTT​TCA​AGA​GGG​TGG​AAC​CTC​TGG-3′ 
14xHis-NEDD8-Tex2(SMP)-F 5′-GGC​GCC​GGT​ACC​TGC​GGA​TCC​GAG​GAG​GAA​GAA​CAG​GAA​GCC​T-3′ 
14xHis-NEDD8-Tex2(SMP)-R 5′-GCT​CAG​CTA​ATT​AAG​AAG​CTT​AGT​GAT​ATA​AAC​ATC​ATC​CAT​GTT​TGG-3′ 
Halo-TMEM55A-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​GCT​GCC​GAG​GGG​GTG-3′ 
Halo-TMEM55A-R 5′-GTA​CCG​TCG​ACT​GCA​GAA​TTC​CTA​TGC​AAA​ACT​GTG​CTC​TGG​ATA​A-3′ 
Halo-TMEM55B-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​GCG​GCA​GAT​GGA​GAG​C-3′ 
Halo-TMEM55B-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TCA​GGA​GAA​GTT​CTG​GAC​AGG​G-3′ 
BFP-TMEM55B-F 5′-TGA​ACC​GTC​AGA​TCC​GCT​AGC​GCC​ACC​ATG​AGC​GAG​CTG​ATT​AAG​GAG​AA-3′ 
BFP-TMEM55B-R 5′-TCC​ATC​TGC​CGC​CAT​GAG​CTC​ATT​AAG​CTT​GTG​CCC​CAG​TTT​G-3′ 
TMEM55B-∆X5R-F 5′-CCT​TAT​CAT​TGC​ATG​CCC​TCG​GCC​CTA​CTG​CA-3′ 
TMEM55B-∆X5R-R 5′-GGC​ATG​CAA​TGA​TAA​GGA​GAC​AGT​TAC​AGG​GGC​A-3′ 
TMEM55B(∆1-74)-F 5′-CTC​GAG​CTC​TAT​TCA​CCC​TTA​ACT​AGC​CCG​GA-3′ 
TMEM55B(∆1-74)-R 5′-GGT​GAA​TAG​AGC​TCG​AGG​GAA​ATC​TCC​AGA​GT-3′ 
TMEM55B- ∆TM-F 5′-TAT​TGG​GCG​CTG​AGG​ATC​CAC​CGG​ATC​TAG​ATA​AC-3′ 
TMEM55B- ∆TM-R 5′-ATC​CTC​AGC​GCC​CAA​TAG​ATG​ACA​CTT​TCC​TG-3′ 
TMEM55B(∆1-163)-F 5′-TTT​CCC​TCG​AGC​TCC​ATC​CCG​GAC​CTC​TGA​GTC​C-3′ 
TMEM55B(∆1-163)-R 5′-ATG​GAG​CTC​GAG​GGA​AAT​CTC​CAG​AGT​AGA​CA-3′ 
TMEM55B-∆PTase(73-163)-F 5′-ACA​TCC​CGG​ACC​TCT​GAG​TCC​AGA​ACC​CCA​AC-3′ 
TMEM55B-∆PTase(73-163)-R 5′-TCA​GAG​GTC​CGG​GAT​GTG​GGT​CCT​CCC​CAG​GCA​AC-3′ 
TMEM55B C140W-F 5′-TCT​GGA​AAG​TGA​CAT​CCC​AAC​GGA​TTG​CAT​GC-3′ 
TMEM55B C140W-R 5′-GGA​TGT​CAC​TTT​CCA​GAT​AAG​GAG​ACA​GTT​ACA​GGG​GCA-3′ 
TMEM55B C140S-F 5′-TCT​CGA​AAG​TGA​CAT​CCC​AAC​GGA​TTG​CAT​GC-3′ 
TMEM55B C140S-R 5′-GGA​TGT​CAC​TTT​CGA​GAT​AAG​GAG​ACA​GTT​ACA​GGG​GCA-3′ 
GST-TMEM55B-F 5′-GAT​CTG​GTT​CCG​CGT​GGA​TCC​ATG​GCG​GCA​GAT​GGA​GAG​C-3′ 
GST-TMEM55B-R 5′-TCA​GTC​AGT​CAC​GAT​GAA​TTC​TCA​GGA​GAA​GTT​CTG​GAC​AGG​G-3′ 
Halo-PI4KIIα-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​GAC​GAG​ACG​AGC​CCA​C-3′ 
Halo-PI4KIIα-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TCA​CCA​CCA​TGA​AAA​GAA​GGG​C-3′ 
Halo-PI4KIIβ-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​GAG​GAT​CCC​TCC​GAG​C-3′ 
Halo-PI4KIIβ-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TCA​CCA​GGA​GGA​AAA​AAA​TGG​C-3′ 
PI4KIIα W359A-F 5′-ACT​CCG​CTA​GGG​CAT​ATC​CTT​TTT​ACT​GGG​CC-3′ 
PI4KIIα W359A-R 5′-ATA​TGC​CCT​AGC​GGA​GTC​AGG​ATG​CTT​CAG​TGG​G-3′ 
RTN4-Halo/GFP-F 5′-TGA​ACC​GTC​AGA​TCC​GCT​AGC​GCC​ACC​ATG​GAA​GAC​CTG​GAC​CAG​TCT​CC-3′ 
RTN4-Halo/GFP-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TTC​AGC​TTT​GCG​CTT​CAA​TCC-3′ 
Halo-ARL6IP1-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​GCG​GAG​GGA​GAT​AAT​CG-3′ 
Halo-ARL6IP1-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TCA​TTC​GTT​TTT​CTT​TTC​TTT​TTG​TT-3′ 
Halo-ATL1-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​GCC​AAG​AAC​CGC​AGG-3′ 
Halo-ATL1-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TTA​CAT​TTT​TTT​CTT​TTC​TGA​TTG​TTC​A-3′ 
Climp63-halo-F 5′-TGA​ACC​GTC​AGA​TCC​GCT​AGC​ATG​CCC​TCG​GCC​AAA​CAA-3′ 
Climp63-halo-R 5′-CGA​CTG​CAG​AAT​TCG​AAG​CTT​GAC​CTT​TTC​GTG​AAT​CTT​CTC​CA-3′ 
Halo-E-Syt1-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​GAG​CGA​TCT​CCA​GGA​GAG-3′ 
GFP-E-Syt1-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​ATG​GAG​CGA​TCT​CCA​GGA​GAG-3′ 
Halo/GFP-E-Syt1-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​CTA​GGA​GCT​GCC​CTT​GTC​CTT-3′ 
Reep5-Halo-F 5′-CCG​GAC​TCA​GAT​CTC​GAG​CTC​GCC​ACC​ATG​TCT​GCG​G-3′ 
Reep5-Halo-R 5′-CGG​TGG​ATC​CCG​GGC​CCG​CGG​GGT​GCT​CTT​CTT​TTC​TTC​ACC​CA-3′ 
MGAT2-Halo-F 5′-TGA​ACC​GTC​AGA​TCC​GCT​AGC​GCC​ACC​ATG​AGG​TTC​CGC​ATC​TAC​AAA​CG-3′ 
MGAT2-Halo-R 5′-CGA​CTG​CAG​AAT​TCG​AAG​CTT​CTG​CAG​TCT​TCT​ATA​ACT​TTT​ACA​GAG​TTC-3′ 
Tex2 KO sgRNA1-F 5′-CAC​CGC​CTC​TGC​ACG​TGC​ACT​TTA​G-3′ 
Tex2 KO sgRNA1-R 5′-AAA​CCT​AAA​GTG​CAC​GTG​CAG​AGG​C-3′ 
Tex2 KO sgRNA2-F 5′-CAC​CGC​AAG​TTG​GCC​ATG​ACC​CCG​C-3′ 
Tex2 KO sgRNA2-R 5′-AAA​CGC​GGG​GTC​ATG​GCC​AAC​TTG​C-3′ 
Tex2 KI sgRNA-F 5′-CAC​CGC​CGG​CAA​TGA​CAA​GTC​TGT​A-3′ 
Tex2 KI sgRNA-R 5′-AAA​CTA​CAG​ACT​TGT​CAT​TGC​CGG​C-3′ 
Tex2 LHA-F 5′-GTC​GAC​GGT​ATC​GAT​AAG​CTT​AGT​CCT​TGG​TAG​AGT​TCT​TGG​TTG​G-3′ 
Tex2 LHA-R 5′-TTT​GCT​CAT​TGC​CGG​CTT​CAC​AAG​GGC-3′ 
sfGFP-F 5′-TGA​AGC​CGG​CAA​TGA​GCA​AAG​GAG​AAG​AAC​TTT​TCA-3′ 
sfGFP-R 5′-GGC​GAC​CAT​ACA​GAC​TTG​TTT​TGT​AGA​GCT​CAT​CCA​TGC​CA-3′ 
Tex2 RHA-F 5′-AAC​AAG​TCT​GTA​TGG​TCG​CCA​TG-3′ 
Tex2 RHA-R 5′-CTA​TAG​GGC​GAA​TTG​GAG​CTC​AGT​ATC​AGA​GGG​AGA​AGT​CAG​TGG​G-3′ 
PrimersSequences (5′-3′)
GFP-Tex2-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​ACA​AGT​CTG​TAT​GGT​CGC​CAT​G-3′ 
GFP-Tex2-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TCA​TGG​CTG​ATC​AGC​AGC​CT-3′ 
GFP-Tex2(1-474)-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​CTA​CTT​CAC​TGG​CAA​TTC​CGG​G-3′ 
GFP-Tex2(1-540)-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TCA​GAT​ATC​CAG​AGA​TCT​TGT​GTT​CC-3′ 
GFP-Tex2(474-1131)-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​ACG​TTG​GGC​TTC​TTT​ATA​ATG​TGT​G-3′ 
GFP-Tex2(1-276)-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​CTA​GGA​ACG​AGT​ATC​AGA​GGG​AGA​A-3′ 
GFP-Tex2(277-517)-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​CGT​TCC​TTT​TTT​AAA​GTG​CCC​G-3′ 
GFP-Tex2(277-517)-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TTA​TGT​AAA​AAA​CCA​AAT​CAC​GCA​A-3′ 
GFP--Tex2(∆ 277-473)-F 5′-GTT​CCT​TTA​CGT​TGG​GCT​TCT​TTA​TAA​TGT​GTG-3′ 
GFP--Tex2(∆ 277-473)-R 5′-GCC​CAA​CGT​AAA​GGA​ACG​AGT​ATC​AGA​GGG​AGA-3′ 
Halo-Tex2-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ACA​AGT​CTG​TAT​GGT​CGC​CAT​G-3′ 
Halo-Tex2-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TCA​TGG​CTG​ATC​AGC​AGC​CT-3′ 
Tex2(TM)-GFP-F 5′-TGA​ACC​GTC​AGA​TCC​GCT​AGC​ACG​TTG​GGC​TTC​TTT​ATA​ATG​TGT​G-3′ 
Tex2(TM)-GFP-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TGT​AAA​AAA​CCA​AAT​CAC​GCA​AA-3′ 
GFP-Tex2(∆ TM)-F 5′-CAG​TGA​AGC​CAC​CAA​GTG​CTC​ATA​AAT​ATC​ACA​AG-3′ 
GFP-Tex2(∆ TM)-R 5′-ACT​TGG​TGG​CTT​CAC​TGG​CAA​TTC​CGG​GTG​CT-3′ 
Halo-Tex2(∆ PH)-F 5′-GAA​GAA​ATC​AAG​AAG​TCA​TCG​GGT​GTC​TCT​GG-3′ 
Halo-Tex2(∆ PH)-R 5′-GAC​TTC​TTG​ATT​TCT​TCT​TTG​ATA​TCC​AGA​GAT​CTT​GTG​TT-3′ 
GFP-Tex2(SMP)-F 5′-CCA​CCC​TCT​ATA​ATG​CAC​TCA​GCC​ATG​GAC​CC-3′ 
GFP-Tex2(SMP)-R 5′-TGC​ATT​ATA​GAG​GGT​GGA​ACC​TCT​GGC​AAC​TT-3′ 
GFP/Halo-Tex2(∆SMP)-F 5′-CCA​CCC​TCt​ATA​ATG​CAC​TCA​GCC​ATG​GAC​CC-3′ 
GFP/Halo-Tex2(∆SMP)-R 5′-TGC​ATT​ATa​GAG​GGT​GGA​ACC​TCT​GGC​AAC​TT-3′ 
GFP-Tex2(∆ TM-∆PH)-F 5′-CAG​TGA​AGG​AGG​AGG​AAG​AAC​AGG​AAG​CCT​GG-3′ 
GFP-Tex2(∆ TM-∆PH)-R 5′-TTC​CTC​CTC​CTT​CAC​TGG​CAA​TTC​CGG​GTG​CT-3′ 
14xHis-NEDD8-Tex2(PH)-F 5′-GGC​GCC​GGT​ACC​TGC​GGA​TCC​CCA​CCA​AGT​GCT​CAT​AAA​TAT​CAC​A-3′ 
14xHis-NEDD8-Tex2(PH)-R 5′-GCT​CAG​CTA​ATT​AAG​AAG​CTT​TCA​AGA​GGG​TGG​AAC​CTC​TGG-3′ 
14xHis-NEDD8-Tex2(SMP)-F 5′-GGC​GCC​GGT​ACC​TGC​GGA​TCC​GAG​GAG​GAA​GAA​CAG​GAA​GCC​T-3′ 
14xHis-NEDD8-Tex2(SMP)-R 5′-GCT​CAG​CTA​ATT​AAG​AAG​CTT​AGT​GAT​ATA​AAC​ATC​ATC​CAT​GTT​TGG-3′ 
Halo-TMEM55A-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​GCT​GCC​GAG​GGG​GTG-3′ 
Halo-TMEM55A-R 5′-GTA​CCG​TCG​ACT​GCA​GAA​TTC​CTA​TGC​AAA​ACT​GTG​CTC​TGG​ATA​A-3′ 
Halo-TMEM55B-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​GCG​GCA​GAT​GGA​GAG​C-3′ 
Halo-TMEM55B-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TCA​GGA​GAA​GTT​CTG​GAC​AGG​G-3′ 
BFP-TMEM55B-F 5′-TGA​ACC​GTC​AGA​TCC​GCT​AGC​GCC​ACC​ATG​AGC​GAG​CTG​ATT​AAG​GAG​AA-3′ 
BFP-TMEM55B-R 5′-TCC​ATC​TGC​CGC​CAT​GAG​CTC​ATT​AAG​CTT​GTG​CCC​CAG​TTT​G-3′ 
TMEM55B-∆X5R-F 5′-CCT​TAT​CAT​TGC​ATG​CCC​TCG​GCC​CTA​CTG​CA-3′ 
TMEM55B-∆X5R-R 5′-GGC​ATG​CAA​TGA​TAA​GGA​GAC​AGT​TAC​AGG​GGC​A-3′ 
TMEM55B(∆1-74)-F 5′-CTC​GAG​CTC​TAT​TCA​CCC​TTA​ACT​AGC​CCG​GA-3′ 
TMEM55B(∆1-74)-R 5′-GGT​GAA​TAG​AGC​TCG​AGG​GAA​ATC​TCC​AGA​GT-3′ 
TMEM55B- ∆TM-F 5′-TAT​TGG​GCG​CTG​AGG​ATC​CAC​CGG​ATC​TAG​ATA​AC-3′ 
TMEM55B- ∆TM-R 5′-ATC​CTC​AGC​GCC​CAA​TAG​ATG​ACA​CTT​TCC​TG-3′ 
TMEM55B(∆1-163)-F 5′-TTT​CCC​TCG​AGC​TCC​ATC​CCG​GAC​CTC​TGA​GTC​C-3′ 
TMEM55B(∆1-163)-R 5′-ATG​GAG​CTC​GAG​GGA​AAT​CTC​CAG​AGT​AGA​CA-3′ 
TMEM55B-∆PTase(73-163)-F 5′-ACA​TCC​CGG​ACC​TCT​GAG​TCC​AGA​ACC​CCA​AC-3′ 
TMEM55B-∆PTase(73-163)-R 5′-TCA​GAG​GTC​CGG​GAT​GTG​GGT​CCT​CCC​CAG​GCA​AC-3′ 
TMEM55B C140W-F 5′-TCT​GGA​AAG​TGA​CAT​CCC​AAC​GGA​TTG​CAT​GC-3′ 
TMEM55B C140W-R 5′-GGA​TGT​CAC​TTT​CCA​GAT​AAG​GAG​ACA​GTT​ACA​GGG​GCA-3′ 
TMEM55B C140S-F 5′-TCT​CGA​AAG​TGA​CAT​CCC​AAC​GGA​TTG​CAT​GC-3′ 
TMEM55B C140S-R 5′-GGA​TGT​CAC​TTT​CGA​GAT​AAG​GAG​ACA​GTT​ACA​GGG​GCA-3′ 
GST-TMEM55B-F 5′-GAT​CTG​GTT​CCG​CGT​GGA​TCC​ATG​GCG​GCA​GAT​GGA​GAG​C-3′ 
GST-TMEM55B-R 5′-TCA​GTC​AGT​CAC​GAT​GAA​TTC​TCA​GGA​GAA​GTT​CTG​GAC​AGG​G-3′ 
Halo-PI4KIIα-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​GAC​GAG​ACG​AGC​CCA​C-3′ 
Halo-PI4KIIα-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TCA​CCA​CCA​TGA​AAA​GAA​GGG​C-3′ 
Halo-PI4KIIβ-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​GAG​GAT​CCC​TCC​GAG​C-3′ 
Halo-PI4KIIβ-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TCA​CCA​GGA​GGA​AAA​AAA​TGG​C-3′ 
PI4KIIα W359A-F 5′-ACT​CCG​CTA​GGG​CAT​ATC​CTT​TTT​ACT​GGG​CC-3′ 
PI4KIIα W359A-R 5′-ATA​TGC​CCT​AGC​GGA​GTC​AGG​ATG​CTT​CAG​TGG​G-3′ 
RTN4-Halo/GFP-F 5′-TGA​ACC​GTC​AGA​TCC​GCT​AGC​GCC​ACC​ATG​GAA​GAC​CTG​GAC​CAG​TCT​CC-3′ 
RTN4-Halo/GFP-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TTC​AGC​TTT​GCG​CTT​CAA​TCC-3′ 
Halo-ARL6IP1-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​GCG​GAG​GGA​GAT​AAT​CG-3′ 
Halo-ARL6IP1-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TCA​TTC​GTT​TTT​CTT​TTC​TTT​TTG​TT-3′ 
Halo-ATL1-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​GCC​AAG​AAC​CGC​AGG-3′ 
Halo-ATL1-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TTA​CAT​TTT​TTT​CTT​TTC​TGA​TTG​TTC​A-3′ 
Climp63-halo-F 5′-TGA​ACC​GTC​AGA​TCC​GCT​AGC​ATG​CCC​TCG​GCC​AAA​CAA-3′ 
Climp63-halo-R 5′-CGA​CTG​CAG​AAT​TCG​AAG​CTT​GAC​CTT​TTC​GTG​AAT​CTT​CTC​CA-3′ 
Halo-E-Syt1-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​GAG​CGA​TCT​CCA​GGA​GAG-3′ 
GFP-E-Syt1-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​ATG​GAG​CGA​TCT​CCA​GGA​GAG-3′ 
Halo/GFP-E-Syt1-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​CTA​GGA​GCT​GCC​CTT​GTC​CTT-3′ 
Reep5-Halo-F 5′-CCG​GAC​TCA​GAT​CTC​GAG​CTC​GCC​ACC​ATG​TCT​GCG​G-3′ 
Reep5-Halo-R 5′-CGG​TGG​ATC​CCG​GGC​CCG​CGG​GGT​GCT​CTT​CTT​TTC​TTC​ACC​CA-3′ 
MGAT2-Halo-F 5′-TGA​ACC​GTC​AGA​TCC​GCT​AGC​GCC​ACC​ATG​AGG​TTC​CGC​ATC​TAC​AAA​CG-3′ 
MGAT2-Halo-R 5′-CGA​CTG​CAG​AAT​TCG​AAG​CTT​CTG​CAG​TCT​TCT​ATA​ACT​TTT​ACA​GAG​TTC-3′ 
Tex2 KO sgRNA1-F 5′-CAC​CGC​CTC​TGC​ACG​TGC​ACT​TTA​G-3′ 
Tex2 KO sgRNA1-R 5′-AAA​CCT​AAA​GTG​CAC​GTG​CAG​AGG​C-3′ 
Tex2 KO sgRNA2-F 5′-CAC​CGC​AAG​TTG​GCC​ATG​ACC​CCG​C-3′ 
Tex2 KO sgRNA2-R 5′-AAA​CGC​GGG​GTC​ATG​GCC​AAC​TTG​C-3′ 
Tex2 KI sgRNA-F 5′-CAC​CGC​CGG​CAA​TGA​CAA​GTC​TGT​A-3′ 
Tex2 KI sgRNA-R 5′-AAA​CTA​CAG​ACT​TGT​CAT​TGC​CGG​C-3′ 
Tex2 LHA-F 5′-GTC​GAC​GGT​ATC​GAT​AAG​CTT​AGT​CCT​TGG​TAG​AGT​TCT​TGG​TTG​G-3′ 
Tex2 LHA-R 5′-TTT​GCT​CAT​TGC​CGG​CTT​CAC​AAG​GGC-3′ 
sfGFP-F 5′-TGA​AGC​CGG​CAA​TGA​GCA​AAG​GAG​AAG​AAC​TTT​TCA-3′ 
sfGFP-R 5′-GGC​GAC​CAT​ACA​GAC​TTG​TTT​TGT​AGA​GCT​CAT​CCA​TGC​CA-3′ 
Tex2 RHA-F 5′-AAC​AAG​TCT​GTA​TGG​TCG​CCA​TG-3′ 
Tex2 RHA-R 5′-CTA​TAG​GGC​GAA​TTG​GAG​CTC​AGT​ATC​AGA​GGG​AGA​AGT​CAG​TGG​G-3′ 
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