Table 1. Antibodies, drugs, siRNAs, and... | Rockefeller University Press

Table 1.

Antibodies, drugs, siRNAs, and polar lipids used in this study

Reagent or Resource	Source	Identifier
Antibodies and reagents
Tex2 Rabbit pAb	ABclonal	A14686
GFP antibody	CST	2555
Anti-Halo Tag pAB	Promega	G928A
His Tag Antibody, mAb, Mouse	Genscript	A00186
GST-Tag	Sinobiological	11213-MM01
Cathepsin D	CST	2284S
Purified Mouse Anti-GM130	BD Biosciences	610822
LAMP-1 (H4A3):	Santa cruz	sc-20011
Calnexin Rabbit pAb	ABclonal	A15631
COXIV Polyclonal antibody	Proteintech	11242-1-AP
β-Tubulin mAB(3G6)	Abbkine	A01030
TMEM55B Polyclonal antibody	Proteintech	23992-1-AP
Thapsigargin	Abcam	ab120286
Lysotracker deep red	Thermo Fisher Scientific	L12492
Bafilomycin A1	MCE	HY-100558
Rapamycin	MCE	HY-10219
siRNA
siRNA human ARL6IP1#1: 5′-GUACAGUUCAAGUGAAUCUGGAUAA-3′	(Dong et al., 2018)	N/A
siRNA human ARL6IP1#2: 5′-UUAUCCAGAUUCACUUGAACUGUACUA-3′	(Dong et al., 2018)	N/A
siRNA human Reep5: 5′-CGAAGAAAGCUACCGUGAATT-3′	(Kumar et al., 2019)	N/A
siRNA human RTN1: 5′-UAGAUGCGGAAACUGAUGGTT-3′	(Anderson and Hetzer, 2008)	N/A
siRNA human RTN3: 5′-CCUUCUAAUUCUUGCUGAATT-3′	(Anderson and Hetzer, 2008)	N/A
siRNA human RTN4#1: 5′-GATCCAAGCTATCCAGAAA-3′	Ribobio	N/A
siRNA human RTN4#2: 5′-GTGTTGATGTGGGTATTTA-3′	Ribobio	N/A
siRNA human RTN4#3: 5′-GGAATCTGAAGTTGCTATA-3′	Ribobio	N/A
siRNA human scrambled siRNA 5′-CGUUAAUCGCGUAUAAUACGCGUAT-3′	Ribobio	N/A
siRNA human PI4KIIα: 5′-GGUUGGUGGUGCUGGAUUATT-3′	Ribobio	N/A
siRNA human PI4KIIβ: 5′-GGUUCAAGUGGAAGUUACUTT-3′	Ribobio	N/A
siRNA human TMEM55B#1: 5′-GCATCAGCATGTAGTCAAA-3′	Ribobio	N/A
siRNA human TMEM55B#2: 5′-GCCAATCTCTCATCAACGT-3′	Ribobio	N/A
siRNA human TMEM55B#3: 5′-GGGCATTTGTCATCCTGTT-3′	Ribobio	N/A
Polar lipids
NBD-PC	Avanti	810133
NBD-PS	Avanti	810198
NBD-PA	Avanti	810138
NBD-PE	Avanti	810144
NBD-Cholesterol	Avanti	810252
NBD-Ceramide	Avanti	810211

Reagent or Resource	Source	Identifier
Antibodies and reagents
Tex2 Rabbit pAb	ABclonal	A14686
GFP antibody	CST	2555
Anti-Halo Tag pAB	Promega	G928A
His Tag Antibody, mAb, Mouse	Genscript	A00186
GST-Tag	Sinobiological	11213-MM01
Cathepsin D	CST	2284S
Purified Mouse Anti-GM130	BD Biosciences	610822
LAMP-1 (H4A3):	Santa cruz	sc-20011
Calnexin Rabbit pAb	ABclonal	A15631
COXIV Polyclonal antibody	Proteintech	11242-1-AP
β-Tubulin mAB(3G6)	Abbkine	A01030
TMEM55B Polyclonal antibody	Proteintech	23992-1-AP
Thapsigargin	Abcam	ab120286
Lysotracker deep red	Thermo Fisher Scientific	L12492
Bafilomycin A1	MCE	HY-100558
Rapamycin	MCE	HY-10219
siRNA
siRNA human ARL6IP1#1: 5′-GUACAGUUCAAGUGAAUCUGGAUAA-3′	(Dong et al., 2018)	N/A
siRNA human ARL6IP1#2: 5′-UUAUCCAGAUUCACUUGAACUGUACUA-3′	(Dong et al., 2018)	N/A
siRNA human Reep5: 5′-CGAAGAAAGCUACCGUGAATT-3′	(Kumar et al., 2019)	N/A
siRNA human RTN1: 5′-UAGAUGCGGAAACUGAUGGTT-3′	(Anderson and Hetzer, 2008)	N/A
siRNA human RTN3: 5′-CCUUCUAAUUCUUGCUGAATT-3′	(Anderson and Hetzer, 2008)	N/A
siRNA human RTN4#1: 5′-GATCCAAGCTATCCAGAAA-3′	Ribobio	N/A
siRNA human RTN4#2: 5′-GTGTTGATGTGGGTATTTA-3′	Ribobio	N/A
siRNA human RTN4#3: 5′-GGAATCTGAAGTTGCTATA-3′	Ribobio	N/A
siRNA human scrambled siRNA 5′-CGUUAAUCGCGUAUAAUACGCGUAT-3′	Ribobio	N/A
siRNA human PI4KIIα: 5′-GGUUGGUGGUGCUGGAUUATT-3′	Ribobio	N/A
siRNA human PI4KIIβ: 5′-GGUUCAAGUGGAAGUUACUTT-3′	Ribobio	N/A
siRNA human TMEM55B#1: 5′-GCATCAGCATGTAGTCAAA-3′	Ribobio	N/A
siRNA human TMEM55B#2: 5′-GCCAATCTCTCATCAACGT-3′	Ribobio	N/A
siRNA human TMEM55B#3: 5′-GGGCATTTGTCATCCTGTT-3′	Ribobio	N/A
Polar lipids
NBD-PC	Avanti	810133
NBD-PS	Avanti	810198
NBD-PA	Avanti	810138
NBD-PE	Avanti	810144
NBD-Cholesterol	Avanti	810252
NBD-Ceramide	Avanti	810211

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