Table 2. Statistics of POI localization... | Rockefeller University Press

Table 2.

Statistics of POI localization counts per centromeric region

POI	Mean count	STD	SEM	Median	Mean POI copy numbers^* (±SE)	FP tag	N
cnp1^CENP-A	46.8	28.0	0.8	40.0	ND	PamCherry1	1,245
cnp20^CENP-T	55.0	32.5	4.3	47.0	61.1 ± 4.8	mEos3.2-A69T	57
fta2^CENP-P	49.6	27.9	3.0	43.0	55.1 ± 3.3	mEos3.2-A69T	87
fta7^CENP-Q	51.7	28.9	3.7	46.0	57.4 ± 4.1	mEos3.2-A69T	61
spc7^KNL1	85.9	48.4	3.0	76.0	95.4 ± 3.3	mEos3.2-A69T	259
nnf1^PMF1	83.3	47.2	3.2	70.0	92.6 ± 3.6	mEos3.2-A69T	217
mis12	65.8	33.1	2.8	57.5	73.1 ± 3.1	mEos3.2-A69T	136
spc25	107.2	52.2	5.6	49.5	119.1 ± 6.2	mEos3.2-A69T	86
ndc80^HEC1	96.5	51.2	3.9	88.0	107.2 ± 4.3	mEos3.2-A69T	172
dam1	57.3	33.1	2.2	49.5	63.7 ± 2.4	mEos3.2-A69T	232

POI	Mean count	STD	SEM	Median	Mean POI copy numbers^* (±SE)	FP tag	N
cnp1^CENP-A	46.8	28.0	0.8	40.0	ND	PamCherry1	1,245
cnp20^CENP-T	55.0	32.5	4.3	47.0	61.1 ± 4.8	mEos3.2-A69T	57
fta2^CENP-P	49.6	27.9	3.0	43.0	55.1 ± 3.3	mEos3.2-A69T	87
fta7^CENP-Q	51.7	28.9	3.7	46.0	57.4 ± 4.1	mEos3.2-A69T	61
spc7^KNL1	85.9	48.4	3.0	76.0	95.4 ± 3.3	mEos3.2-A69T	259
nnf1^PMF1	83.3	47.2	3.2	70.0	92.6 ± 3.6	mEos3.2-A69T	217
mis12	65.8	33.1	2.8	57.5	73.1 ± 3.1	mEos3.2-A69T	136
spc25	107.2	52.2	5.6	49.5	119.1 ± 6.2	mEos3.2-A69T	86
ndc80^HEC1	96.5	51.2	3.9	88.0	107.2 ± 4.3	mEos3.2-A69T	172
dam1	57.3	33.1	2.2	49.5	63.7 ± 2.4	mEos3.2-A69T	232

Distributions can be found in Fig. 2 D and E. N, number of centromeric regions analyzed.

Mean POI copy numbers were derived from the mean localization counts of each POI using 0.9 as a calibration factor as determined by the FtnA protein standard (Materials and methods).

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