IANBD-cTnCT53C fluorescence lifetime changes with low and high Ca2+
| Experiment (N) . | Buffer condition . | Buffer condition . | Average lifetime . | SD . | C.V. . | n . | Change + Ca2+ . | Z′ . | P . |
|---|---|---|---|---|---|---|---|---|---|
| #1 | Rigor | Low Ca2+ | 2.61 | 0.01 | 0.4% | 23 | |||
| Rigor | High Ca2+ | 2.42 | 0.02 | 0.4% | 23 | −7.5% | 0.70 | 4.6 × 10−46 | |
| #2 | Rigor | Low Ca2+ | 2.67 | 0.02 | 0.7% | 23 | |||
| Rigor | High Ca2+ | 2.42 | 0.01 | 0.5% | 23 | −9.4% | 0.64 | 3.2 × 10−42 | |
| #3 | Rigor | Low Ca2+ | 2.69 | 0.01 | 0.5% | 24 | |||
| Rigor | High Ca2+ | 2.39 | 0.02 | 0.8% | 24 | −11.1% | 0.65 | 4.1 × 10−45 | |
| #4 | Rigor | Low Ca2+ | 2.62 | 0.01 | 0.4% | 12 | |||
| Rigor | High Ca2+ | 2.37 | 0.02 | 0.7% | 12 | −9.6% | 0.68 | 3.1 × 10−23 | |
| Average | Rigor | Low Ca2+ | − | − | − | ||||
| Rigor | High Ca2+ | − | − | − | −9.4% | 0.67 | 7.7 × 10−24 |
| Experiment (N) . | Buffer condition . | Buffer condition . | Average lifetime . | SD . | C.V. . | n . | Change + Ca2+ . | Z′ . | P . |
|---|---|---|---|---|---|---|---|---|---|
| #1 | Rigor | Low Ca2+ | 2.61 | 0.01 | 0.4% | 23 | |||
| Rigor | High Ca2+ | 2.42 | 0.02 | 0.4% | 23 | −7.5% | 0.70 | 4.6 × 10−46 | |
| #2 | Rigor | Low Ca2+ | 2.67 | 0.02 | 0.7% | 23 | |||
| Rigor | High Ca2+ | 2.42 | 0.01 | 0.5% | 23 | −9.4% | 0.64 | 3.2 × 10−42 | |
| #3 | Rigor | Low Ca2+ | 2.69 | 0.01 | 0.5% | 24 | |||
| Rigor | High Ca2+ | 2.39 | 0.02 | 0.8% | 24 | −11.1% | 0.65 | 4.1 × 10−45 | |
| #4 | Rigor | Low Ca2+ | 2.62 | 0.01 | 0.4% | 12 | |||
| Rigor | High Ca2+ | 2.37 | 0.02 | 0.7% | 12 | −9.6% | 0.68 | 3.1 × 10−23 | |
| Average | Rigor | Low Ca2+ | − | − | − | ||||
| Rigor | High Ca2+ | − | − | − | −9.4% | 0.67 | 7.7 × 10−24 |
Average data are provided for individual experiments. Experiments were carried out with three separate protein preparations of troponin that was exchanged into four separate myofibril preparations. Low Ca2+ is pCa 9 and High Ca2+ is pCa 4.5. The unit for Average (fluorescence) lifetime and SD (standard deviation) is nanoseconds. n = number of wells of myofibrils into which low or high Ca2+ is individually added. Change + Ca2+ is the percentage change in lifetime between low and high Ca2+ in each experiment. C.V. is the coefficient of variance. Statistical tests of Z′ factor and t test are used to evaluate the change in lifetime between low and high Ca2+. The average Z′ and percentage Change + Ca2+ for the four experiments is also given.