Table 1. IANBD-cTnC T53C fluorescence... | Rockefeller University Press

Table 1.

IANBD-cTnC^T53C fluorescence intensity changes with low and high Ca²⁺

Experiment (N)	Buffer condition	Buffer condition	Average intensity	SD	C.V.	n	Change + Ca²⁺	Z′	P
#1	Rigor	Low Ca²⁺	130	9	6.6%	23
	Rigor	High Ca²⁺	117	10	8.9%	21	−9.5%	−3.6	1.0 × 10^-4
#2	Rigor	Low Ca²⁺	129	17	13.4%	24
	Rigor	High Ca²⁺	123	16	13.4%	23	−4.8%	−15.3	0.215
#3	Rigor	Low Ca²⁺	135	26	19.3%	23
	Rigor	High Ca²⁺	111	27	24.2%	23	−17.5%	−5.7	0.004
#4	Rigor	Low Ca²⁺	141	7	5.2%	12
	Rigor	High Ca²⁺	129	16	12.5%	12	−8.1%	−5.2	0.036
Average	Rigor	Low Ca²⁺	−	−	−
	Rigor	High Ca²⁺	−	−	−		−10.0%	−7.5	0.06

Experiment (N)	Buffer condition	Buffer condition	Average intensity	SD	C.V.	n	Change + Ca²⁺	Z′	P
#1	Rigor	Low Ca²⁺	130	9	6.6%	23
	Rigor	High Ca²⁺	117	10	8.9%	21	−9.5%	−3.6	1.0 × 10^-4
#2	Rigor	Low Ca²⁺	129	17	13.4%	24
	Rigor	High Ca²⁺	123	16	13.4%	23	−4.8%	−15.3	0.215
#3	Rigor	Low Ca²⁺	135	26	19.3%	23
	Rigor	High Ca²⁺	111	27	24.2%	23	−17.5%	−5.7	0.004
#4	Rigor	Low Ca²⁺	141	7	5.2%	12
	Rigor	High Ca²⁺	129	16	12.5%	12	−8.1%	−5.2	0.036
Average	Rigor	Low Ca²⁺	−	−	−
	Rigor	High Ca²⁺	−	−	−		−10.0%	−7.5	0.06

Average data are provided for individual experiments. Experiments were carried out with three separate protein preparations of troponin that was exchanged into four separate myofibril preparations. Low Ca²⁺ is pCa 9 and High Ca²⁺ is pCa 4.5. The unit for Average (fluorescence) intensity of IANBD-cTnC^T53C and SD is millivolts. n = number of wells of myofibrils into which low or high Ca²⁺ is individually added. Change + Ca²⁺ is the percentage change in intensity between low and high Ca²⁺ in each experiment. C.V. is the coefficient of variance. Statistical tests of Z′ factor and t test are used to evaluate the change in intensity between low and high Ca²⁺. The average Z′ and percentage Change + Ca²⁺ for the four experiments is also given.

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