P values for data in Fig. 6
| Log [ACh] (M): . | −9 . | −8.5 . | −8 . | −7.5 . | −7 . | −6.5 . | −6 . | −5.5 . | −5 . |
|---|---|---|---|---|---|---|---|---|---|
| Veh vs. L-NNA, Indo | 0.6386 | 0.1156 | 0.091 | 0.0012 | 0.0169 | 0.0097 | 0.0441 | 0.0426 | 0.0432 |
| All in the presence of L-NNA, Indo | |||||||||
| Veh vs. PcTX1 | 0.9697 | 0.3630 | 0.9712 | 0.4479 | 0.9744 | 0.6251 | 0.0184 | 0.0086 | 0.0116 |
| Veh vs. Tram/AP | 0.7449 | 0.5454 | 0.9996 | 0.5537 | 0.5960 | 0.3092 | 0.0426 | 0.0437 | 0.0169 |
| PcTX1 vs. Tram/AP | 0.7875 | 0.9744 | 0.9909 | 0.9752 | 0.5357 | 0.6873 | 0.9069 | 0.4692 | 0.7627 |
| Repeated-measures, BL vs. 10−9to 10−5[ACh] (indicates [ACh] affect on diameter) | |||||||||
| Vehicle | 0.8836 | 0.8285 | 0.4802 | 0.0145 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 |
| L-NNA, Indo | 0.2808 | 0.6559 | 0.6334 | 0.9374 | 0.5581 | 0.0130 | 0.0122 | 0.0232 | 0.0285 |
| L-NNA, Indo, PcTX1 | 0.4846 | 0.3019 | 0.9983 | 0.9022 | 0.7495 | 0.2803 | 0.5386 | 0.9878 | 0.8413 |
| L-NNA, Indo, Tram/AP | 0.9370 | 0.9979 | 0.9880 | 0.9998 | 0.9932 | 0.1773 | 0.1307 | 0.3101 | 0.3972 |
| Log [ACh] (M): . | −9 . | −8.5 . | −8 . | −7.5 . | −7 . | −6.5 . | −6 . | −5.5 . | −5 . |
|---|---|---|---|---|---|---|---|---|---|
| Veh vs. L-NNA, Indo | 0.6386 | 0.1156 | 0.091 | 0.0012 | 0.0169 | 0.0097 | 0.0441 | 0.0426 | 0.0432 |
| All in the presence of L-NNA, Indo | |||||||||
| Veh vs. PcTX1 | 0.9697 | 0.3630 | 0.9712 | 0.4479 | 0.9744 | 0.6251 | 0.0184 | 0.0086 | 0.0116 |
| Veh vs. Tram/AP | 0.7449 | 0.5454 | 0.9996 | 0.5537 | 0.5960 | 0.3092 | 0.0426 | 0.0437 | 0.0169 |
| PcTX1 vs. Tram/AP | 0.7875 | 0.9744 | 0.9909 | 0.9752 | 0.5357 | 0.6873 | 0.9069 | 0.4692 | 0.7627 |
| Repeated-measures, BL vs. 10−9to 10−5[ACh] (indicates [ACh] affect on diameter) | |||||||||
| Vehicle | 0.8836 | 0.8285 | 0.4802 | 0.0145 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 |
| L-NNA, Indo | 0.2808 | 0.6559 | 0.6334 | 0.9374 | 0.5581 | 0.0130 | 0.0122 | 0.0232 | 0.0285 |
| L-NNA, Indo, PcTX1 | 0.4846 | 0.3019 | 0.9983 | 0.9022 | 0.7495 | 0.2803 | 0.5386 | 0.9878 | 0.8413 |
| L-NNA, Indo, Tram/AP | 0.9370 | 0.9979 | 0.9880 | 0.9998 | 0.9932 | 0.1773 | 0.1307 | 0.3101 | 0.3972 |
Data represent biological replicates and were analyzed by two-way ANOVA. The two-way ANOVA revealed that there was a statistically significant interaction (P < 0.0001) between factors. Post hoc pairwise comparisons were analyzed between individual groups using Tukey’s multiple comparisons test. P < 0.05 (bold text) is considered significantly different.