List of primary and secondary antibodies used for immunofluorescence
| Antibody . | Company, Cat. # . | RRID . | Host; clone . | Dilution . | Fig. # . | Reference . |
|---|---|---|---|---|---|---|
| Primary | ||||||
| CD31 | Abcam, ab124432 | AB_2802125 | Rabbit; poly | 1:250 | Fig. 1 | |
| αSMA | Sigma-Aldrich, A2547 | AB_476701 | Mouse; mono | 1:300 | Fig. 1 | |
| ASIC1 (E−15) | Santa Cruz Biotechnology, sc-13903 | AB_633515 | Goat; poly | 1:50 | Figs. 1, 5, and 6 | Nitta et al., 2014 |
| ASIC1 | Millipore Sigma, AB5674P | AB_91972 | Rabbit; poly | 1:100 | Fig. 6 E | Jernigan et al., 2009 |
| STIM1 (44/GOK) | BD Biosciences, 610954 | AB_398267 | Mouse; mono | 1:200 | Fig. 5 | Krishnan et al., 2022 |
| IK1 (D-5) | Santa Cruz Biotechnology, sc-365265 | AB_10841432 | Mouse; mono | 1:50 | Fig. 6 | Lu et al., 2017 |
| KCNN3 (KCa2.3) | Alomone Labs, APC-025 | AB_10613112 | Rabbit; poly | 1:200 | Fig. 6 | Kim et al., 2012 |
| Secondary | ||||||
| Alexa Fluor 488 anti-rabbit IgG (H + L) | Jackson ImmunoResearch Laboratories, Inc 711-546-152 | AB_2340619 | Donkey; poly | 1:500 | Fig. 1 | |
| Cy3 AffiniPure anti-goat IgG (H + L) | Jackson ImmunoResearch Laboratories, Inc 705-165-147 | AB_2307351 | Donkey; poly | 1:500 | Figs. 1 and 5 C | |
| Alexa Fluor 647 anti-mouse IgG (H + L) | Jackson ImmunoResearch Laboratories, Inc 715-605-150 | AB_2340862 | Donkey; poly | 1:500 | Figs. 1 and 5 C | |
| Alexa Fluor 647 anti-rabbit IgG (H + L) | Jackson ImmunoResearch Laboratories, Inc 711-605-152 | AB_2492288 | Donkey; poly | 1:500 | Fig. 5 D | |
| Antibody . | Company, Cat. # . | RRID . | Host; clone . | Dilution . | Fig. # . | Reference . |
|---|---|---|---|---|---|---|
| Primary | ||||||
| CD31 | Abcam, ab124432 | AB_2802125 | Rabbit; poly | 1:250 | Fig. 1 | |
| αSMA | Sigma-Aldrich, A2547 | AB_476701 | Mouse; mono | 1:300 | Fig. 1 | |
| ASIC1 (E−15) | Santa Cruz Biotechnology, sc-13903 | AB_633515 | Goat; poly | 1:50 | Figs. 1, 5, and 6 | Nitta et al., 2014 |
| ASIC1 | Millipore Sigma, AB5674P | AB_91972 | Rabbit; poly | 1:100 | Fig. 6 E | Jernigan et al., 2009 |
| STIM1 (44/GOK) | BD Biosciences, 610954 | AB_398267 | Mouse; mono | 1:200 | Fig. 5 | Krishnan et al., 2022 |
| IK1 (D-5) | Santa Cruz Biotechnology, sc-365265 | AB_10841432 | Mouse; mono | 1:50 | Fig. 6 | Lu et al., 2017 |
| KCNN3 (KCa2.3) | Alomone Labs, APC-025 | AB_10613112 | Rabbit; poly | 1:200 | Fig. 6 | Kim et al., 2012 |
| Secondary | ||||||
| Alexa Fluor 488 anti-rabbit IgG (H + L) | Jackson ImmunoResearch Laboratories, Inc 711-546-152 | AB_2340619 | Donkey; poly | 1:500 | Fig. 1 | |
| Cy3 AffiniPure anti-goat IgG (H + L) | Jackson ImmunoResearch Laboratories, Inc 705-165-147 | AB_2307351 | Donkey; poly | 1:500 | Figs. 1 and 5 C | |
| Alexa Fluor 647 anti-mouse IgG (H + L) | Jackson ImmunoResearch Laboratories, Inc 715-605-150 | AB_2340862 | Donkey; poly | 1:500 | Figs. 1 and 5 C | |
| Alexa Fluor 647 anti-rabbit IgG (H + L) | Jackson ImmunoResearch Laboratories, Inc 711-605-152 | AB_2492288 | Donkey; poly | 1:500 | Fig. 5 D | |