Table 1.

Percent populations of myosin active states by thick filament zone

Untreated (Fig. 3 d)
% (±SEM) P-zone C-zone D-zone 
Activea 11.1 (±0.8) 7.4 (±0.5) 8.8 (±0.8) 
DRX 54 (±2.5) 40.1 (±1) 47.7 (±1.9) 
SRX 35 (±5.5) 52.5 (±4) 43.6 (±5.4) 
Mava (Fig. 4 c ) 
Activea 12.8 (±1.3) 6.7 (±0.4) 6 (±0.2)b 
DRX 53.9 (±3.7) 33.4 (±1.1)b 30.6 (±0.6)b 
SRX 33.3 (±10.8) 59.9 (±3.6) 63.4 (±2.7)b 
PKA (Fig. 4 d ) 
Activea 10.3 (±0.7) 5.7 (±0.3)b 8.1 (±0.4) 
DRX 67.1 (±1.6)b 60 (±1.4)b 47 (±0.9) 
SRX 22.6 (±6.6) 34.3 (±3.3)b 44.9 (±2.7) 
Change from untreated to mava (Fig. 4 a ) 
Activea 1.7 (±1.6) −0.7 (±0.6) −2.8 (±0.8) 
DRX −0.1 (±4.5) −6.7 (±1.5) −17.1 (±2) 
SRX −1.6 (±12.2) 7.4 (±5.4) 19.8 (±6.1) 
Change from untreated to PKA (Fig. 4 b ) 
Activea −0.8 (±1.1) −1.7 (±0.6) −0.7 (±0.8) 
DRX 13.1 (±3) 19.9 (±1.7) −0.7 (±2.1) 
SRX −12.4 (±8.6) −18.1 (±5.2) 1.4 (±6) 
SRX:DRX ratio (Fig. 4 e ) 
Untreated 0.6 (±0.1) 1.3 (±0.1) 0.9 (±0.1) 
+Mava 0.6 (±0.2) 1.8 (±0.1)b 2.1 (±0.1)b 
+PKA 0.3 (±0.1) 0.6 (±0.1)b 1.0 (±0.1) 
Untreated (Fig. 3 d)
% (±SEM) P-zone C-zone D-zone 
Activea 11.1 (±0.8) 7.4 (±0.5) 8.8 (±0.8) 
DRX 54 (±2.5) 40.1 (±1) 47.7 (±1.9) 
SRX 35 (±5.5) 52.5 (±4) 43.6 (±5.4) 
Mava (Fig. 4 c ) 
Activea 12.8 (±1.3) 6.7 (±0.4) 6 (±0.2)b 
DRX 53.9 (±3.7) 33.4 (±1.1)b 30.6 (±0.6)b 
SRX 33.3 (±10.8) 59.9 (±3.6) 63.4 (±2.7)b 
PKA (Fig. 4 d ) 
Activea 10.3 (±0.7) 5.7 (±0.3)b 8.1 (±0.4) 
DRX 67.1 (±1.6)b 60 (±1.4)b 47 (±0.9) 
SRX 22.6 (±6.6) 34.3 (±3.3)b 44.9 (±2.7) 
Change from untreated to mava (Fig. 4 a ) 
Activea 1.7 (±1.6) −0.7 (±0.6) −2.8 (±0.8) 
DRX −0.1 (±4.5) −6.7 (±1.5) −17.1 (±2) 
SRX −1.6 (±12.2) 7.4 (±5.4) 19.8 (±6.1) 
Change from untreated to PKA (Fig. 4 b ) 
Activea −0.8 (±1.1) −1.7 (±0.6) −0.7 (±0.8) 
DRX 13.1 (±3) 19.9 (±1.7) −0.7 (±2.1) 
SRX −12.4 (±8.6) −18.1 (±5.2) 1.4 (±6) 
SRX:DRX ratio (Fig. 4 e ) 
Untreated 0.6 (±0.1) 1.3 (±0.1) 0.9 (±0.1) 
+Mava 0.6 (±0.2) 1.8 (±0.1)b 2.1 (±0.1)b 
+PKA 0.3 (±0.1) 0.6 (±0.1)b 1.0 (±0.1) 
a

Active does not refer to active myosins necessarily. Instead, this simply refers to the fastest fitted phase which is likely dominated by nonspecific immobilizations of Cy3-ATP.

b

Statistically significantly different from untreated (unpaired two-tail t test, P < 0.05).

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