Table 1. Primer sequences.
Primer nameSequence
BamHI-ATAT1(M1)-Fw 5′-ATA​TGG​ATC​CAT​GGA​GTT​CCC​GTT​CGA​TGT​G-3′ 
ATAT1(P284)-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​GGG​GCG​GAG​GGG​ACC​TCG​TTC-3′ 
ATAT1(S315)-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​tta​GCT​GCC​CCC​AGG​GTC​AGC​AGC​CAA​CAG​AAG​GCG​GGC​GGT​AGG​GTG-3′ 
ATAT1(NLS R/A)-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​TGC​GGT​AGC​AGC​AGC​TTG​AGC​TGG​GCT​GCC​CCC​AGG​GTC​AGC​AGC-3′ 
ATAT1(NES VL/A)-Re 5′-GCA​TGC​GCG​TGC​GGA​ACG​TGC​TGC​CTC​CTG​CTC​TGG​TGC​AAA​GGG​GCG​GAG​GGG​ACC​TCG-3′ 
ATAT1(NES VL/A)-Fw 5′-GCA​CCA​GAG​CAG​GAG​GCA​GCA​CGT​TCC​GCA​CGC​GCA​TGC​CCC​CCA​CAC​CCT​ACC​GCC-3′ 
ATAT1(S236)-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​GGA​GTA​TGG​CTT​GAT​GTC​TCC-3′ 
ATAT1(D157N)-Re 5′-CAG​CAG​CTT​CTG​TGA​GGG​TCG​GTT​AAT​TGC​CAG​TTG​GTG​CGG​TTC-3′ 
ATAT1(D157N)-Fw 5′-GAA​CCG​CAC​CAA​CTG​GCA​ATT​AAC​CGA​CCC​TCA​CAG​AAG​CTG​CTG-3′ 
ATAT1(S315D,T322E)-Re 5′-TAT​GCG​GCC​GCT​TAC​CTT​TCG​CGA​CGA​CGT​TGA​GCT​GGA​TCG​CCC​CCA​GGG​TCA​GCA​GCC-3′ 
ATAT1(S315A,T322A)-Re 5′-TAT​GCG​GCC​GCT​TAC​CTC​GCG​CGA​CGA​CGT​TGA​GCT​GGC​GCG​CCC​CCA​GGG​TCA​GCA​GCC-3′ 
BamHI-GS-ATAT1(S236)-Fw 5′-ATA​TGG​ATC​CGG​ATC​TTC​TAG​TGA​CCG​AGA​ATT​TCT​G-3′ 
BamHI-GS-ATAT1(L282)-Fw 5′-ATA​TGG​ATC​CGG​ATC​TCT​CCG​CCC​CTT​TGT​GCC​AGA​G-3′ 
ATAT1(L/F -> A)-Re 5′-TGC​TGC​CTC​CTG​CTC​TGG​TGC​TGC​GGG​GCG​TGC​GGG​ACC​TCG​TTC​TGG​TGA​GTT​TC-3′ 
ATAT1(T322A)-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​CCT​GGC​GCG​ACG​ACG​TTG​AGC​TGG​GCT​G-3′ 
ATAT1(T322E)-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​CCT​TTC​GCG​ACG​ACG​TTG​AGC​TGG​GCT​G-3′ 
ATAT1(S315A)-Stop-NotI-Re 5′-TAT​GCG​GCC​GCT​TAC​CTG​GTG​CGA​CGA​CGT​TGA​GCT​GGG​GCG​CCC​CCA​GGG​TCA​GCA​GCC-3′ 
ATAT1(S315D)-Stop-NotI-Re 5′-TATGCGGCCGCTTACCTGGTGCGACGACGTTGAGCTGGDTCGCCCCCAGGGTCAGCAGCC-3′ 
ATAT1(V286, L291-2/A)-Fw 5′-GCA​CCA​GAG​CAG​GAG​GCA​GCA​CGT​TCC​TTG​CGC​CTC​TGC​CCC​CCA​CAC-3′ 
ATAT1(R321)-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​GCG​ACG​ACG​TTG​AGC​TGG​GCT​G-3′ 
BamHI-GS-KPNA4-Fw 5′-ATA​TGG​ATC​CGG​ATC​TGC​GGA​CAA​CGA​GAA​ACT​GGA​CAA​C-3′ 
BamHI-GS-14-3-3-β-Fw 5′-ATA​TGG​ATC​CGG​TTC​AAT​GAC​AAT​GGA​TAA​AAG​TGA​G-3′ 
14-3-3-β-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​GTT​CTC​TCC​CTC​CCC​AGC​GTC​TCC​TTC​GTC​TCC​CTG​GTT​TTC-3′ 
BamHI-GS-14-3-3-γ-Fw 5′-ATA​TGG​ATC​CGG​TTC​AAT​GGT​GGA​CCG​CGA​GCA​ACT​G-3′ 
14-3-3-γ-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​ATT​GTT​GCC​TTC​GCC​GCC​ATC-3′ 
BamHI-GS-14-3-3-ε-Fw 5′-ATA​TGG​ATC​CGG​TTC​AAT​GGA​TGA​TCG​GGA​GGA​TCT​G-3′ 
BamHI-GS-14-3-3-η-Fw 5′-ATA​TGG​ATC​CGG​TTC​AAT​GGG​GGA​CCG​GGA​GCA​GCT​G-3′ 
14-3-3-η-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TCA​GTT​GCC​TTC​TCC​TGC​TTC​TTC-3′ 
BamHI-GS-14-3-3-θ-Fw 5′-ATA​TGG​ATC​CGG​TTC​AAT​GGA​GAA​GAC​TGA​GCT​GAT​C-3′ 
14-3-3-θ-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​GTT​TTC​AGC​CCC​TTC​TGC​CGC​ATC​ACA​TTC​TTC​TCC​TGC​ACT​GTC​TGA​TGT​CCA​AAG-3′ 
Cdk1(D146N)-Re 5′-CCA​AAA​GCT​CTG​GCA​AGG​CCA​AAA​TtA​GCC​AGT​TTA​ATT​GTT​CCT​TTG​TC-3′ 
Cdk1(D146N)-Fw 5′-GAC​AAA​GGA​ACA​ATT​AAA​CTG​GCT​aAT​TTT​GGC​CTT​GCC​AGA​GCT​TTT​GG-3′ 
BamHI-Kozak-Cdk1(M1)-Fw 5′-ATA​TGG​ATC​CGC​CAC​CAT​GGA​AGA​TTA​TAC​CAA​AAT​AGA​G-3′ 
Cdk1-NotI-Re 5′-ATA​TGC​GGC​CGC​ACA​TCT​TCT​TAA​TCT​GAT​TGT​C-3′ 
BamHI-GS-CK2alpha(M1)-Fw 5′-ATA​TGG​ATC​CGG​TTC​CAT​GTC​GGG​ACC​CGT​GCC​AAG​C-3′ 
CK2alpha(Q391)-Stop-Re 5′-ATA​TGC​GGC​CGC​TTA​CTG​CTG​AGC​GCC​AGC​GGC​AGC-3′ 
CK2alpha(K68A)-Re 5′-CTT​CTT​TTT​TAC​TGG​CTT​GAG​AAT​CGC​AAC​AAC​AAC​TTT​TTC​ATT​ATT​TG-3′ 
CK2alpha(K68A)-Fw 5′-CAA​ATA​ATG​AAA​AAG​TTG​TTG​TTG​CGA​TTC​TCA​AGC​CAG​TAA​AAA​AGA​AG-3′ 
BamHI-GS-PPP2CA(M1)-Fw 5′-ATA​TGG​ATC​CGG​TTC​AAT​GGA​CGA​GAA​GGT​GTT​CAC​CAA​G-3′ 
PPP2CA-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​CAG​GAA​GTA​GTC​TGG​GGT​ACG​ACG-3′ 
BamHI-R18-Fw 5′-ATA​TGG​ATC​CCC​TCA​CTG​CGT​GCC​CAG​AGA​C-3′ 
DIFOPEIN1-Re 5′-GTG​AGG​AGC​TCC​ATC​AGC​ACT​GTC​GAG​TCC​TGC​TGC​TCC​GGG​CAG​GCA​CAT​GTT​GGC​CTC-3′ 
DIFOPEIN2-Re1 5′-TGG​CCT​CCA​GGT​CCA​GCC​AGC​TCA​GGT​CTG​GGC​ACG​CAG​TGA​GGA​GCT​CCA​TCA​GCA​C-3′ 
DIFOPEIN3-EcoRI-Re 5′-ATA​TGA​ATT​CCA​GTC​CTG​CTG​CTC​CAG​AGG​GCA​GGC​ACA​TGT​TGG​CCT​CCA​GGT​CCA​GCC-3′ 
Primer nameSequence
BamHI-ATAT1(M1)-Fw 5′-ATA​TGG​ATC​CAT​GGA​GTT​CCC​GTT​CGA​TGT​G-3′ 
ATAT1(P284)-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​GGG​GCG​GAG​GGG​ACC​TCG​TTC-3′ 
ATAT1(S315)-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​tta​GCT​GCC​CCC​AGG​GTC​AGC​AGC​CAA​CAG​AAG​GCG​GGC​GGT​AGG​GTG-3′ 
ATAT1(NLS R/A)-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​TGC​GGT​AGC​AGC​AGC​TTG​AGC​TGG​GCT​GCC​CCC​AGG​GTC​AGC​AGC-3′ 
ATAT1(NES VL/A)-Re 5′-GCA​TGC​GCG​TGC​GGA​ACG​TGC​TGC​CTC​CTG​CTC​TGG​TGC​AAA​GGG​GCG​GAG​GGG​ACC​TCG-3′ 
ATAT1(NES VL/A)-Fw 5′-GCA​CCA​GAG​CAG​GAG​GCA​GCA​CGT​TCC​GCA​CGC​GCA​TGC​CCC​CCA​CAC​CCT​ACC​GCC-3′ 
ATAT1(S236)-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​GGA​GTA​TGG​CTT​GAT​GTC​TCC-3′ 
ATAT1(D157N)-Re 5′-CAG​CAG​CTT​CTG​TGA​GGG​TCG​GTT​AAT​TGC​CAG​TTG​GTG​CGG​TTC-3′ 
ATAT1(D157N)-Fw 5′-GAA​CCG​CAC​CAA​CTG​GCA​ATT​AAC​CGA​CCC​TCA​CAG​AAG​CTG​CTG-3′ 
ATAT1(S315D,T322E)-Re 5′-TAT​GCG​GCC​GCT​TAC​CTT​TCG​CGA​CGA​CGT​TGA​GCT​GGA​TCG​CCC​CCA​GGG​TCA​GCA​GCC-3′ 
ATAT1(S315A,T322A)-Re 5′-TAT​GCG​GCC​GCT​TAC​CTC​GCG​CGA​CGA​CGT​TGA​GCT​GGC​GCG​CCC​CCA​GGG​TCA​GCA​GCC-3′ 
BamHI-GS-ATAT1(S236)-Fw 5′-ATA​TGG​ATC​CGG​ATC​TTC​TAG​TGA​CCG​AGA​ATT​TCT​G-3′ 
BamHI-GS-ATAT1(L282)-Fw 5′-ATA​TGG​ATC​CGG​ATC​TCT​CCG​CCC​CTT​TGT​GCC​AGA​G-3′ 
ATAT1(L/F -> A)-Re 5′-TGC​TGC​CTC​CTG​CTC​TGG​TGC​TGC​GGG​GCG​TGC​GGG​ACC​TCG​TTC​TGG​TGA​GTT​TC-3′ 
ATAT1(T322A)-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​CCT​GGC​GCG​ACG​ACG​TTG​AGC​TGG​GCT​G-3′ 
ATAT1(T322E)-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​CCT​TTC​GCG​ACG​ACG​TTG​AGC​TGG​GCT​G-3′ 
ATAT1(S315A)-Stop-NotI-Re 5′-TAT​GCG​GCC​GCT​TAC​CTG​GTG​CGA​CGA​CGT​TGA​GCT​GGG​GCG​CCC​CCA​GGG​TCA​GCA​GCC-3′ 
ATAT1(S315D)-Stop-NotI-Re 5′-TATGCGGCCGCTTACCTGGTGCGACGACGTTGAGCTGGDTCGCCCCCAGGGTCAGCAGCC-3′ 
ATAT1(V286, L291-2/A)-Fw 5′-GCA​CCA​GAG​CAG​GAG​GCA​GCA​CGT​TCC​TTG​CGC​CTC​TGC​CCC​CCA​CAC-3′ 
ATAT1(R321)-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​GCG​ACG​ACG​TTG​AGC​TGG​GCT​G-3′ 
BamHI-GS-KPNA4-Fw 5′-ATA​TGG​ATC​CGG​ATC​TGC​GGA​CAA​CGA​GAA​ACT​GGA​CAA​C-3′ 
BamHI-GS-14-3-3-β-Fw 5′-ATA​TGG​ATC​CGG​TTC​AAT​GAC​AAT​GGA​TAA​AAG​TGA​G-3′ 
14-3-3-β-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​GTT​CTC​TCC​CTC​CCC​AGC​GTC​TCC​TTC​GTC​TCC​CTG​GTT​TTC-3′ 
BamHI-GS-14-3-3-γ-Fw 5′-ATA​TGG​ATC​CGG​TTC​AAT​GGT​GGA​CCG​CGA​GCA​ACT​G-3′ 
14-3-3-γ-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​ATT​GTT​GCC​TTC​GCC​GCC​ATC-3′ 
BamHI-GS-14-3-3-ε-Fw 5′-ATA​TGG​ATC​CGG​TTC​AAT​GGA​TGA​TCG​GGA​GGA​TCT​G-3′ 
BamHI-GS-14-3-3-η-Fw 5′-ATA​TGG​ATC​CGG​TTC​AAT​GGG​GGA​CCG​GGA​GCA​GCT​G-3′ 
14-3-3-η-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TCA​GTT​GCC​TTC​TCC​TGC​TTC​TTC-3′ 
BamHI-GS-14-3-3-θ-Fw 5′-ATA​TGG​ATC​CGG​TTC​AAT​GGA​GAA​GAC​TGA​GCT​GAT​C-3′ 
14-3-3-θ-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​GTT​TTC​AGC​CCC​TTC​TGC​CGC​ATC​ACA​TTC​TTC​TCC​TGC​ACT​GTC​TGA​TGT​CCA​AAG-3′ 
Cdk1(D146N)-Re 5′-CCA​AAA​GCT​CTG​GCA​AGG​CCA​AAA​TtA​GCC​AGT​TTA​ATT​GTT​CCT​TTG​TC-3′ 
Cdk1(D146N)-Fw 5′-GAC​AAA​GGA​ACA​ATT​AAA​CTG​GCT​aAT​TTT​GGC​CTT​GCC​AGA​GCT​TTT​GG-3′ 
BamHI-Kozak-Cdk1(M1)-Fw 5′-ATA​TGG​ATC​CGC​CAC​CAT​GGA​AGA​TTA​TAC​CAA​AAT​AGA​G-3′ 
Cdk1-NotI-Re 5′-ATA​TGC​GGC​CGC​ACA​TCT​TCT​TAA​TCT​GAT​TGT​C-3′ 
BamHI-GS-CK2alpha(M1)-Fw 5′-ATA​TGG​ATC​CGG​TTC​CAT​GTC​GGG​ACC​CGT​GCC​AAG​C-3′ 
CK2alpha(Q391)-Stop-Re 5′-ATA​TGC​GGC​CGC​TTA​CTG​CTG​AGC​GCC​AGC​GGC​AGC-3′ 
CK2alpha(K68A)-Re 5′-CTT​CTT​TTT​TAC​TGG​CTT​GAG​AAT​CGC​AAC​AAC​AAC​TTT​TTC​ATT​ATT​TG-3′ 
CK2alpha(K68A)-Fw 5′-CAA​ATA​ATG​AAA​AAG​TTG​TTG​TTG​CGA​TTC​TCA​AGC​CAG​TAA​AAA​AGA​AG-3′ 
BamHI-GS-PPP2CA(M1)-Fw 5′-ATA​TGG​ATC​CGG​TTC​AAT​GGA​CGA​GAA​GGT​GTT​CAC​CAA​G-3′ 
PPP2CA-Stop-NotI-Re 5′-ATA​TGC​GGC​CGC​TTA​CAG​GAA​GTA​GTC​TGG​GGT​ACG​ACG-3′ 
BamHI-R18-Fw 5′-ATA​TGG​ATC​CCC​TCA​CTG​CGT​GCC​CAG​AGA​C-3′ 
DIFOPEIN1-Re 5′-GTG​AGG​AGC​TCC​ATC​AGC​ACT​GTC​GAG​TCC​TGC​TGC​TCC​GGG​CAG​GCA​CAT​GTT​GGC​CTC-3′ 
DIFOPEIN2-Re1 5′-TGG​CCT​CCA​GGT​CCA​GCC​AGC​TCA​GGT​CTG​GGC​ACG​CAG​TGA​GGA​GCT​CCA​TCA​GCA​C-3′ 
DIFOPEIN3-EcoRI-Re 5′-ATA​TGA​ATT​CCA​GTC​CTG​CTG​CTC​CAG​AGG​GCA​GGC​ACA​TGT​TGG​CCT​CCA​GGT​CCA​GCC-3′ 
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