Table 1. HS binding scFv antibodies and... | Rockefeller University Press

Table 1.

HS binding scFv antibodies and their characteristics

scFv name	V_H family	CDR3 sequence	HS modifications required for binding^{^a}
AO4B08	DP-47	SLRMNGWRAHQ	N-Sulfation, C5-epimerization, 2-O-Sulfation, 6-O-Sulfation
EW3D10	DP-38	GRTVGRN	N-Sulfation, 6-O-sulfation
EW3F5	DP-38	SGRQARQGRFPK	Unknown
EW4E9	DP-38	LRGTKMFRH	Unknown
HS3A8	DP-38	GMRPRL	N-sulfation, C5-epimerization, 2-O-sulfation, 6-O-sulfation (likely)
HS4D4	DP-58	GMRPRL	N-sulfation/N-Acetylated, C5-epimerization, 2-O-sulfation (likely), 6-O-sulfation (likely)
HS4E4	DP-38	HAPLRNTRTNT	N-sulfation/N-Acetylated, C5-epimerization, 2-O-sulfation, 6-O-sulfation
LKIV69	DP-38	GSRSSR	N-Sulfation, C5-epimerization, 2-O-Sulfation
MPB49^{^b}	DP-38	WRNDRQ	No known epitope (negative control)

scFv name	V_H family	CDR3 sequence	HS modifications required for binding^{^a}
AO4B08	DP-47	SLRMNGWRAHQ	N-Sulfation, C5-epimerization, 2-O-Sulfation, 6-O-Sulfation
EW3D10	DP-38	GRTVGRN	N-Sulfation, 6-O-sulfation
EW3F5	DP-38	SGRQARQGRFPK	Unknown
EW4E9	DP-38	LRGTKMFRH	Unknown
HS3A8	DP-38	GMRPRL	N-sulfation, C5-epimerization, 2-O-sulfation, 6-O-sulfation (likely)
HS4D4	DP-58	GMRPRL	N-sulfation/N-Acetylated, C5-epimerization, 2-O-sulfation (likely), 6-O-sulfation (likely)
HS4E4	DP-38	HAPLRNTRTNT	N-sulfation/N-Acetylated, C5-epimerization, 2-O-sulfation, 6-O-sulfation
LKIV69	DP-38	GSRSSR	N-Sulfation, C5-epimerization, 2-O-Sulfation
MPB49^{^b}	DP-38	WRNDRQ	No known epitope (negative control)

Heparan sulfate modifications required for scFv binding. CDR3 is the complementarity determining region three within the variable heavy chain.

Compiled from Attreed et al. (2016).

MPB49 has no known epitope, does not appear to bind HS, and serves as a negative control.