Table 1.

Constructs

PrimerSequence 5′ to 3′
QG37LA forward primer TAG​ACT​ACT​AGC​ATC​ACG​GAA​AC 
QG37LA reverse primer ATG​AGT​TTG​GAT​GCA​TCT​ATG 
TG57SA forward primer CTG​CAA​TAC​ACA​AAA​AGT​GCA​ATT​TTT​TTT​C 
TG57SA reverse primer TTC​CCG​TAT​GCT​ATT​GTC​AAC​CA 
G112A G113A forward primer TAT​CTG​CGG​CGG​ACA​TTC​CCA​G 
G112A G113A reverse primer GTT​GAC​ACT​CAG​GAA​CAG​CA 
SG131TA forward primer GAT​ACA​GCA​GAC​ATT​TGC​TCA​GA 
SG131TA reverse primer TTG​TGC​CTC​TGA​AGA​TTC​CTG​C 
G166E forward primer GGC​CTG​AAG​AAA​ATG​TGG​ATG 
G166E reverse primer CCA​GAA​ACT​GAC​GAT​TTT​TAT​AAT​C 
PrimerSequence 5′ to 3′
QG37LA forward primer TAG​ACT​ACT​AGC​ATC​ACG​GAA​AC 
QG37LA reverse primer ATG​AGT​TTG​GAT​GCA​TCT​ATG 
TG57SA forward primer CTG​CAA​TAC​ACA​AAA​AGT​GCA​ATT​TTT​TTT​C 
TG57SA reverse primer TTC​CCG​TAT​GCT​ATT​GTC​AAC​CA 
G112A G113A forward primer TAT​CTG​CGG​CGG​ACA​TTC​CCA​G 
G112A G113A reverse primer GTT​GAC​ACT​CAG​GAA​CAG​CA 
SG131TA forward primer GAT​ACA​GCA​GAC​ATT​TGC​TCA​GA 
SG131TA reverse primer TTG​TGC​CTC​TGA​AGA​TTC​CTG​C 
G166E forward primer GGC​CTG​AAG​AAA​ATG​TGG​ATG 
G166E reverse primer CCA​GAA​ACT​GAC​GAT​TTT​TAT​AAT​C 
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