Abundance of select ciliary membrane proteins in control and arl13 samples
| . | WT . | arl13 . | ||||||
|---|---|---|---|---|---|---|---|---|
| Coverage (%) . | Total peptides . | Unique peptides . | Coverage (%) . | Total peptides . | Unique peptides . | |||
| Protein (ID #) | N-terminus | Exp.1/Exp.2/Exp.3 (average ±) | Exp.1/Exp.2/Exp.3 (average ±) | Exp.1/Exp.2/Exp.3 (average ±) | Exp.1/Exp.2/Exp.3 (average ±) | Exp.1/Exp.2/Exp.3 (average ±) | Exp.1/Exp.2/Exp.3 (average ±) | |
| Accumulated | PLD | mgcasskeev | 0/0/26.9 (9.0 ± 15.54) | 0/0/7 (2.3 ± 4.04) | 0/0/4 (1.33 ± 2.31) | 52.9/78.9/57.4 (63.1 ± 13.90) | 11/40/35 (28.7 ± 15.50) | 10/18/12 (13.3 ± 4.16) |
| AMPK | mgaccsqpse | 0/0/7.5 (2.5 ± 4.3) | 0/0/5 (1.7 ± 2.9) | 0/0/3 (1 ± 1.7) | 32.5/42.1/45.0 (39.8 ± 6.6) | 16/34/49 (33 ± 16.5) | 14/17/16 (15.7 ± 1.5) | |
| PLC | mgnvfscfet | 0/0/0 (0) | 0/0/0 (0) | 0/0/0 (0) | 30.5/45.3/30.9 (35.6 ± 8.4) | 22/40/41 (34.3 ± 10.7) | 16/18/14 (16 ± 2) | |
| CDPKK1 | mgcvgskeda | 0/0/0 (0) | 0/0/0 (0) | 0/0/0 (0) | 13/15.3/20.1 (16.1 ± 3.7) | 8/24/28 (20 ± 10.6) | 8/11/13 (10.7 ± 2.5) | |
| Reduced | FAP12 | mgcgasvmnr | 78.2/80.6/72.0 (76.9 ± 4.4) | 154/384/462 (333.3 ± 160.1) | 53/52/47 (50.7 ± 3.2) | 47.8/77.6/71.6 (65.7 ± 15.8) | 37/132/289 (127.3 ± 152.7) | 27/41/44 (37.3 ± 9.1) |
| FAP138 | mgcgasvmnr | 35.2/41.4/28.1 (34.9 ± 6.6) | 10/21/13 (14.7 ± 5.7) | 7/9/6 (7.3 ± 1.5) | 13.6/0/9.9 (7.8 ± 7.02) | 4/0/4 (2.3 ± 2.7) | 3/0/2 (1.7 ± 1.5) | |
| FAP124 | mskrtadgst | 24.4/25.9/33.1 (27.8 ± 4.6) | 21/49/56 (42 ± 18.5) | 21/22/27 (23.3 ± 3.2) | 0/0/0 (0) | 0/0/0 (0) | 0/0/0 (0) | |
| PTP1 | mgsgastqvq | 15.9/10.2/7.1 (11.1 ± 4.5) | 12/10/11 (11 ± 1) | 9/6/4 (6.3 ± 2.5) | 0/0/0 (0) | 0/0/0 (0) | 0/0/0 (0) | |
| . | WT . | arl13 . | ||||||
|---|---|---|---|---|---|---|---|---|
| Coverage (%) . | Total peptides . | Unique peptides . | Coverage (%) . | Total peptides . | Unique peptides . | |||
| Protein (ID #) | N-terminus | Exp.1/Exp.2/Exp.3 (average ±) | Exp.1/Exp.2/Exp.3 (average ±) | Exp.1/Exp.2/Exp.3 (average ±) | Exp.1/Exp.2/Exp.3 (average ±) | Exp.1/Exp.2/Exp.3 (average ±) | Exp.1/Exp.2/Exp.3 (average ±) | |
| Accumulated | PLD | mgcasskeev | 0/0/26.9 (9.0 ± 15.54) | 0/0/7 (2.3 ± 4.04) | 0/0/4 (1.33 ± 2.31) | 52.9/78.9/57.4 (63.1 ± 13.90) | 11/40/35 (28.7 ± 15.50) | 10/18/12 (13.3 ± 4.16) |
| AMPK | mgaccsqpse | 0/0/7.5 (2.5 ± 4.3) | 0/0/5 (1.7 ± 2.9) | 0/0/3 (1 ± 1.7) | 32.5/42.1/45.0 (39.8 ± 6.6) | 16/34/49 (33 ± 16.5) | 14/17/16 (15.7 ± 1.5) | |
| PLC | mgnvfscfet | 0/0/0 (0) | 0/0/0 (0) | 0/0/0 (0) | 30.5/45.3/30.9 (35.6 ± 8.4) | 22/40/41 (34.3 ± 10.7) | 16/18/14 (16 ± 2) | |
| CDPKK1 | mgcvgskeda | 0/0/0 (0) | 0/0/0 (0) | 0/0/0 (0) | 13/15.3/20.1 (16.1 ± 3.7) | 8/24/28 (20 ± 10.6) | 8/11/13 (10.7 ± 2.5) | |
| Reduced | FAP12 | mgcgasvmnr | 78.2/80.6/72.0 (76.9 ± 4.4) | 154/384/462 (333.3 ± 160.1) | 53/52/47 (50.7 ± 3.2) | 47.8/77.6/71.6 (65.7 ± 15.8) | 37/132/289 (127.3 ± 152.7) | 27/41/44 (37.3 ± 9.1) |
| FAP138 | mgcgasvmnr | 35.2/41.4/28.1 (34.9 ± 6.6) | 10/21/13 (14.7 ± 5.7) | 7/9/6 (7.3 ± 1.5) | 13.6/0/9.9 (7.8 ± 7.02) | 4/0/4 (2.3 ± 2.7) | 3/0/2 (1.7 ± 1.5) | |
| FAP124 | mskrtadgst | 24.4/25.9/33.1 (27.8 ± 4.6) | 21/49/56 (42 ± 18.5) | 21/22/27 (23.3 ± 3.2) | 0/0/0 (0) | 0/0/0 (0) | 0/0/0 (0) | |
| PTP1 | mgsgastqvq | 15.9/10.2/7.1 (11.1 ± 4.5) | 12/10/11 (11 ± 1) | 9/6/4 (6.3 ± 2.5) | 0/0/0 (0) | 0/0/0 (0) | 0/0/0 (0) | |
Summary of mass spectrometry analyses of isolated matrix fractions. Only proteins, which were enriched or reduced in all three replicates are shown. Peptide coverage, total peptides identified, number of unique peptides identified, and the corresponding averages across three experiments are listed. PLD, phospholipase D (Cre13.g591900); AMPK, AMP-regulated kinase (Cre16.g657350); PLC, phosphatidylinositol phospholipase C (Cre06.g270200); CDPKK1: calcium/calmodulin-dependent protein kinase kinase (Cre10.g428650); FAP12: flagellar associated protein 12 (Cre09.g390615); FAP138: flagellar associated protein 138 (Cre14.g632350); FAP124: flagellar associated protein 124 (Cre09.g386400); PTP1: protein tyrosine phosphatase 1 (Cre07.g325724).