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Table 1.
Single-molecule detachment forces
Kinesin motorCOS-7 cell lysate (pN)E. coli expressed (pN)
Kinesin-1 KIF5C(1–560)C 4.4 (3.8, 4.9) ND 
Kinesin-3 UNC-104(1–389)E ND 2.4 (2.0, 2.7) 
KIF1A(1–393) 2.7 (2.3, 3.0) 2.7 (2.3, 3.1) 
KIF1A(1–393)-SWE ND 2.0 (1.6, 2.3) 
KIF1A(1–393)-SWAE ND 1.8 (1.5, 2.1) 
KIF1A(1–393)-V8M 2.0 (1.7, 2.2) 1.9 (1.7, 2.2) 
KIF1A(1–393)-Y89D 1.0 (0.9, 1.2) 1.0 (0.9, 1.2) 
KIF1A(FLAct)C 2.8 (2.4, 3.3) ND 
KIF1A(FLAct)-V8MC 1.8 (1.5, 2.1) ND 
KIF1A(FLAct)-Y89DC 1.1 (0.9, 1.3) ND 
Kinesin motorCOS-7 cell lysate (pN)E. coli expressed (pN)
Kinesin-1 KIF5C(1–560)C 4.4 (3.8, 4.9) ND 
Kinesin-3 UNC-104(1–389)E ND 2.4 (2.0, 2.7) 
KIF1A(1–393) 2.7 (2.3, 3.0) 2.7 (2.3, 3.1) 
KIF1A(1–393)-SWE ND 2.0 (1.6, 2.3) 
KIF1A(1–393)-SWAE ND 1.8 (1.5, 2.1) 
KIF1A(1–393)-V8M 2.0 (1.7, 2.2) 1.9 (1.7, 2.2) 
KIF1A(1–393)-Y89D 1.0 (0.9, 1.2) 1.0 (0.9, 1.2) 
KIF1A(FLAct)C 2.8 (2.4, 3.3) ND 
KIF1A(FLAct)-V8MC 1.8 (1.5, 2.1) ND 
KIF1A(FLAct)-Y89DC 1.1 (0.9, 1.3) ND 

Data are reported as median (quartiles). C, COS-7 expression; E, E. coli expression.

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