| Glucose status . | Low glucose . | High glucose . | |||||
|---|---|---|---|---|---|---|---|
| . | Cell 1 . | Cell 2 . | Cell 3 . | Cell 1 . | Cell 2 . | Cell 3 . | Cell 4 . |
| Cell volume (µm3) | 799 | 997 | 927 | 1,010 | 834 | 898 | 793 |
| Nucleus volume (µm3) | 115 | 150 | 148 | 126 | 116 | 102 | 106 |
| Nucleus volume (%) | 14.40 | 15.00 | 16.00 | 12.50 | 13.90 | 11.40 | 13.40 |
| Mitochondria total volume (µm3) | 73 | 50 | 104 | 68 | 82 | 44 | 98 |
| Mitochondria total volume (%) | 9.10 | 5.00 | 11.20 | 6.70 | 9.90 | 4.90 | 12.40 |
| Golgi volume (µm3) | 18 | 8 | 8 | 22 | 18 | 12 | 13 |
| Golgi volume (%) | 2.20 | 0.80 | 0.90 | 2.20 | 2.10 | 1.30 | 1.60 |
| Insulin SG number | 5,139 | 9,947 | 4,073 | 9,714 | 7,289 | 13,812 | 9,310 |
| Insulin SG total volume (µm3) | 133 | 210 | 108 | 208 | 153 | 254 | 152 |
| Insulin SG total volume (%) | 16.60 | 21.00 | 11.60 | 20.60 | 18.40 | 28.30 | 19.10 |
| Insulin SG average volume (µm3) | 0.026 | 0.021 | 0.027 | 0.022 | 0.021 | 0.019 | 0.016 |
| Insulin SG average surface area (µm2) | 0.419 | 0.367 | 0.426 | 0.369 | 0.366 | 0.337 | 0.309 |
| Insulin SG average diameter (µm) | 0.409 | 0.383 | 0.413 | 0.384 | 0.384 | 0.368 | 0.355 |
| Insulin SG assoc. with MT in % of all SG | 30.70 | 34.40 | 37.30 | 29.10 | 39.00 | 28.70 | 27.00 |
| Assoc. insulin SG close to PM (≤500 nm) in % | 53.00 | 45.30 | 59.20 | 56.60 | 55.40 | 51.20 | 62.10 |
| Not assoc. insulin SG close to PM (≤500 nm) in % | 50.60 | 44.40 | 51.50 | 41.70 | 49.50 | 25.30 | 59.60 |
| Microtubule number | 342 | 291 | 440 | 1,007 | 1,101 | 862 | 932 |
| Microtubule average length (µm) | 8.82 | 14.63 | 8.54 | 3.1 | 3.14 | 3.25 | 2.66 |
| Microtubule cumulative length (µm) | 3,018 | 4,260 | 3,756 | 3,115 | 3,452 | 2,799 | 2,479 |
| Microtubule average tortuosity | 1.05 | 1.04 | 1.04 | 1.05 | 1.05 | 1.04 | 1.05 |
| Microtubule centriole-connected % | 3.50 | 2.70 | 3.90 | 0.70 | 1.70 | 0.70 | 1.30 |
| Microtubule Golgi-connected % | 9.60 | 0.30 | 0.50 | 3.70 | 10.20 | 3.00 | 3.20 |
| Glucose status . | Low glucose . | High glucose . | |||||
|---|---|---|---|---|---|---|---|
| . | Cell 1 . | Cell 2 . | Cell 3 . | Cell 1 . | Cell 2 . | Cell 3 . | Cell 4 . |
| Cell volume (µm3) | 799 | 997 | 927 | 1,010 | 834 | 898 | 793 |
| Nucleus volume (µm3) | 115 | 150 | 148 | 126 | 116 | 102 | 106 |
| Nucleus volume (%) | 14.40 | 15.00 | 16.00 | 12.50 | 13.90 | 11.40 | 13.40 |
| Mitochondria total volume (µm3) | 73 | 50 | 104 | 68 | 82 | 44 | 98 |
| Mitochondria total volume (%) | 9.10 | 5.00 | 11.20 | 6.70 | 9.90 | 4.90 | 12.40 |
| Golgi volume (µm3) | 18 | 8 | 8 | 22 | 18 | 12 | 13 |
| Golgi volume (%) | 2.20 | 0.80 | 0.90 | 2.20 | 2.10 | 1.30 | 1.60 |
| Insulin SG number | 5,139 | 9,947 | 4,073 | 9,714 | 7,289 | 13,812 | 9,310 |
| Insulin SG total volume (µm3) | 133 | 210 | 108 | 208 | 153 | 254 | 152 |
| Insulin SG total volume (%) | 16.60 | 21.00 | 11.60 | 20.60 | 18.40 | 28.30 | 19.10 |
| Insulin SG average volume (µm3) | 0.026 | 0.021 | 0.027 | 0.022 | 0.021 | 0.019 | 0.016 |
| Insulin SG average surface area (µm2) | 0.419 | 0.367 | 0.426 | 0.369 | 0.366 | 0.337 | 0.309 |
| Insulin SG average diameter (µm) | 0.409 | 0.383 | 0.413 | 0.384 | 0.384 | 0.368 | 0.355 |
| Insulin SG assoc. with MT in % of all SG | 30.70 | 34.40 | 37.30 | 29.10 | 39.00 | 28.70 | 27.00 |
| Assoc. insulin SG close to PM (≤500 nm) in % | 53.00 | 45.30 | 59.20 | 56.60 | 55.40 | 51.20 | 62.10 |
| Not assoc. insulin SG close to PM (≤500 nm) in % | 50.60 | 44.40 | 51.50 | 41.70 | 49.50 | 25.30 | 59.60 |
| Microtubule number | 342 | 291 | 440 | 1,007 | 1,101 | 862 | 932 |
| Microtubule average length (µm) | 8.82 | 14.63 | 8.54 | 3.1 | 3.14 | 3.25 | 2.66 |
| Microtubule cumulative length (µm) | 3,018 | 4,260 | 3,756 | 3,115 | 3,452 | 2,799 | 2,479 |
| Microtubule average tortuosity | 1.05 | 1.04 | 1.04 | 1.05 | 1.05 | 1.04 | 1.05 |
| Microtubule centriole-connected % | 3.50 | 2.70 | 3.90 | 0.70 | 1.70 | 0.70 | 1.30 |
| Microtubule Golgi-connected % | 9.60 | 0.30 | 0.50 | 3.70 | 10.20 | 3.00 | 3.20 |
Volumes of the analyzed cells, nuclei, mitochondria, Golgi apparati, insulin SGs. Insulin SG numbers and individual volumes. Numbers and percentages of microtubule-associated and –not associated SGs. Numbers, average and cumulative lengths, and tortuosity of microtubules. Percentages of centrosomal and Golgi-connected microtubules. assoc., associated; MT, microtubule; PM, plasma membrane.