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Table I.

Bud scar distribution in cells containing different alleles of RGA1 or its GAP domain fused to BUD3, MLC2, or CYK3

StrainsPercentage of cells with

Zero bud scars
One bud scar
Two bud scars
Three bud scars
Four bud scars
HA-RGA1 40.0 20.0 12.5 11.0 16.5 
HA-rga1R829A 72.5 22.5 4.0 1.0 0.0 
GFP-RGA1 38.0 24.5 12.5 16.5 8.5 
GFP-rga1700–1007aa rga1Δ∷HIS3 68.0 21.0 8.5 2.0 0.5 
rga1Δ∷HIS3 BUD3-GFP 76.5 17.5 5.5 0.5 0.0 
rga1Δ∷HIS3 BUD3-rga1700–1007aa-GFP 43.5 26.0 12.5 11.5 6.5 
rga1Δ∷HIS3 BUD3-rga1700–1007aa,R829A-GFP 68.5 24.5 4.5 2.0 0.5 
rga1Δ∷HIS3 BUD3-rga2712–1009aa-GFP 68.0 26.0 4.0 1.0 1.0 
rga1Δ∷HIS3 BUD3-bem3818–1128aa-GFP 77.0 15.0 6.0 1.0 1.0 
rga1Δ∷HIS3 MLC2-GFP 62.5 23.5 12.0 2.0 0.0 
rga1Δ∷HIS3 MLC2-rga1700–1007aa-GFP 53.5 24.5 16.0 4.0 2.0 
rga1Δ∷HIS3 CYK3-GFP 71.0 20.5 7.5 1.0 0.0 
rga1Δ∷HIS3 CYK3-rga1700–1007aa-GFP 38.5 17.0 22.0 13.5 9.0 
StrainsPercentage of cells with

Zero bud scars
One bud scar
Two bud scars
Three bud scars
Four bud scars
HA-RGA1 40.0 20.0 12.5 11.0 16.5 
HA-rga1R829A 72.5 22.5 4.0 1.0 0.0 
GFP-RGA1 38.0 24.5 12.5 16.5 8.5 
GFP-rga1700–1007aa rga1Δ∷HIS3 68.0 21.0 8.5 2.0 0.5 
rga1Δ∷HIS3 BUD3-GFP 76.5 17.5 5.5 0.5 0.0 
rga1Δ∷HIS3 BUD3-rga1700–1007aa-GFP 43.5 26.0 12.5 11.5 6.5 
rga1Δ∷HIS3 BUD3-rga1700–1007aa,R829A-GFP 68.5 24.5 4.5 2.0 0.5 
rga1Δ∷HIS3 BUD3-rga2712–1009aa-GFP 68.0 26.0 4.0 1.0 1.0 
rga1Δ∷HIS3 BUD3-bem3818–1128aa-GFP 77.0 15.0 6.0 1.0 1.0 
rga1Δ∷HIS3 MLC2-GFP 62.5 23.5 12.0 2.0 0.0 
rga1Δ∷HIS3 MLC2-rga1700–1007aa-GFP 53.5 24.5 16.0 4.0 2.0 
rga1Δ∷HIS3 CYK3-GFP 71.0 20.5 7.5 1.0 0.0 
rga1Δ∷HIS3 CYK3-rga1700–1007aa-GFP 38.5 17.0 22.0 13.5 9.0 

200 cells were counted for each strain, excluding unbudded daughter cells. The cells with only one chitin ring at the base of a growing bud were counted as having zero bud scars.

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