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Table 1.
Plus end dynamic instability parameters for full-length wild-type tubulins and chimeric tubulins
Total tubulin concentration and recombinant proteinPolymerization rate (subunit · s−1)Catastrophe frequency (min1)
Mean ± SDnMean ± SDn
6 μM     
 α/βIIB 9 ± 2 70 0.06 ± 0.008 60 
 α/βIIB-tail-III 6 ± 1 87 0.08 ± 0.008 92 
 α/βIII-tail-IIB 8 ± 2 37 0.12 ± 0.02 58 
 α/βIII* 8 ± 3 77 0.10 ± 0.01 105 
10.5 μM     
 α/βIIB 15 ± 3 70 0.03 ± 0.006 33 
 α/βIIB-tail-III 14 ± 3 81 0.03 ± 0.005 37 
 α/βIII-tail-IIB 17 ± 2 88 0.10 ± 0.01 115 
 α/βIII* 16 ± 6 75 0.10 ± 0.01 104 
 Mixed (3:1) (α/βIIB:α/βIII) ND  0.05 ± 0.008 41 
 Mixed (1:1) (α/βIIB:α/βIII) 14 ± 2 79 0.06 ± 0.007 65 
 Mixed (1:3) (α/βIIB:α/βIII) ND  0.12 ± 0.009 153 
13 μM     
 α/βIIB 25 ± 4 52 0.03 ± 0.006 20 
 α/βIIB-tail-III 21 ± 3 49 0.008 ± 0.003 
 α/βIII-tail-IIB 19 ± 3 53 0.08 ± 0.01 62 
 α/βIII* 19 ± 4 57 0.09 ± 0.01 68 
Total tubulin concentration and recombinant proteinPolymerization rate (subunit · s−1)Catastrophe frequency (min1)
Mean ± SDnMean ± SDn
6 μM     
 α/βIIB 9 ± 2 70 0.06 ± 0.008 60 
 α/βIIB-tail-III 6 ± 1 87 0.08 ± 0.008 92 
 α/βIII-tail-IIB 8 ± 2 37 0.12 ± 0.02 58 
 α/βIII* 8 ± 3 77 0.10 ± 0.01 105 
10.5 μM     
 α/βIIB 15 ± 3 70 0.03 ± 0.006 33 
 α/βIIB-tail-III 14 ± 3 81 0.03 ± 0.005 37 
 α/βIII-tail-IIB 17 ± 2 88 0.10 ± 0.01 115 
 α/βIII* 16 ± 6 75 0.10 ± 0.01 104 
 Mixed (3:1) (α/βIIB:α/βIII) ND  0.05 ± 0.008 41 
 Mixed (1:1) (α/βIIB:α/βIII) 14 ± 2 79 0.06 ± 0.007 65 
 Mixed (1:3) (α/βIIB:α/βIII) ND  0.12 ± 0.009 153 
13 μM     
 α/βIIB 25 ± 4 52 0.03 ± 0.006 20 
 α/βIIB-tail-III 21 ± 3 49 0.008 ± 0.003 
 α/βIII-tail-IIB 19 ± 3 53 0.08 ± 0.01 62 
 α/βIII* 19 ± 4 57 0.09 ± 0.01 68 

Measurements are from data presented in Fig. 2 (F, G, N, and O) and Fig. 4 (I and J). Mean ± SD are shown. n represents the number of filaments analyzed (for polymerization rate) or catastrophe events (for catastrophe frequency). *, data from Ti et al. (2016); ND, not determined.

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