List of primers used in this study
| ID | Sequence 5′ to 3′ | Restriction sites |
| FLAG-S | GATCTGATTACAAGGATGACGACGATAAGCTCGAGGGCGGCCGCGGAA | BglII, XhoI, NotI, and HindIII |
| FLAG-AS | AGCTTTCCGCGGCCGCCCTCGAGCTTATCGTCGTCATCCTTGTAATCA | BglII, XhoI, NotI, and HindIII |
| Ku70-F | CCGCTCGAGTCAGGGTGGGAGTCATATTACAAAACC | XhoI |
| Ku70-R | GGCGGATCCTTAGTCCTGGAAGTGCTTGGTGAGG | BamHI |
| Ku80-F | GGCCTCGAGGTGCGGTCGGGGAATAAGG | XhoI |
| Ku80-R | CGCGGATCCTATCATGTCCAATAAATCGTCCACATCACC | BamHI |
| XRCC4-F | CCGCTCGAGGACTACAAGGACGATGACGACAAGGGATCC | XhoI |
| XRCC4-R | GGCGAATTCTTAAATCTCATCAAAGAGGTCTTCTGG | EcoRI |
| AAVMCS-F | CGCGCGGCCGCGCTAGCGAATTCGCAGCTGTTGTGCTGTGTATGG | NotI, NheI, and EcoRI |
| AAVMCS-R | CGCGGGCCCACGCGTGGATCCCATCAACAGCATTCAACTGTGCC | BamHI, MluI, and Bsp12OI |
| Ku70-HA1-F | CGCGCGGCCGCGAAGACTGCTTGGTGAATTCTGGAGTTCGG | NotI |
| Ku70-HA1-R | GCCGCTAGCCATGTTGGCTACTGCTCACTAGGCG | NheI |
| Ku70-GFP-F | GCCGCTAGCAAGGGCGAGGAGCTGTTCACCG | NheI |
| Ku70-GFP-R | GGCACCGGTCTTATCGTCGTCATCCTTGTAATCAG | AgeI |
| Ku70-HA2-F | CCGACCGGTTCAGGGTGGGAGTCATATTACAAAACC | AgeI |
| Ku70-HA2-R | CCGACGCGTCAGTGAACCGAGATTGAGTCAGTGC | MluI |
| GFP-F | GGCAAGCTTACCGGTCGCCACCATGGTGAGCAAGGGCG | HindIII and AgeI |
| GFP-R | CCGCTCGAGCTTATCGTCGTCATCC | XhoI |
| Ku70siR-R | GGCACGCGTGCGGCCGCTTAGTCCTGGAAGTGCTTGGTGAGG | MluI and NotI |
| Ku70Mut6E1-R | GCTCTATTGGAGGAGGTTCGAGAGCCTTCTGGACCAGATTATAAATGC | − |
| Ku70Mut6E2-F | GGTCCAGAAGGCTCTCGAACCTCCTCCAATAGAGCTCTATCGG | − |
| Ku70Mut6E2-R | GGAGGGATGTTCCTCTCGGGTGTGTATCTGCACAATGCTGC | − |
| Ku70Mut6E3-F | GCAGATACACACCCGAGAGGAACATCCCTCCTTATTTTGTGGC | − |
| ID | Sequence 5′ to 3′ | Restriction sites |
| FLAG-S | GATCTGATTACAAGGATGACGACGATAAGCTCGAGGGCGGCCGCGGAA | BglII, XhoI, NotI, and HindIII |
| FLAG-AS | AGCTTTCCGCGGCCGCCCTCGAGCTTATCGTCGTCATCCTTGTAATCA | BglII, XhoI, NotI, and HindIII |
| Ku70-F | CCGCTCGAGTCAGGGTGGGAGTCATATTACAAAACC | XhoI |
| Ku70-R | GGCGGATCCTTAGTCCTGGAAGTGCTTGGTGAGG | BamHI |
| Ku80-F | GGCCTCGAGGTGCGGTCGGGGAATAAGG | XhoI |
| Ku80-R | CGCGGATCCTATCATGTCCAATAAATCGTCCACATCACC | BamHI |
| XRCC4-F | CCGCTCGAGGACTACAAGGACGATGACGACAAGGGATCC | XhoI |
| XRCC4-R | GGCGAATTCTTAAATCTCATCAAAGAGGTCTTCTGG | EcoRI |
| AAVMCS-F | CGCGCGGCCGCGCTAGCGAATTCGCAGCTGTTGTGCTGTGTATGG | NotI, NheI, and EcoRI |
| AAVMCS-R | CGCGGGCCCACGCGTGGATCCCATCAACAGCATTCAACTGTGCC | BamHI, MluI, and Bsp12OI |
| Ku70-HA1-F | CGCGCGGCCGCGAAGACTGCTTGGTGAATTCTGGAGTTCGG | NotI |
| Ku70-HA1-R | GCCGCTAGCCATGTTGGCTACTGCTCACTAGGCG | NheI |
| Ku70-GFP-F | GCCGCTAGCAAGGGCGAGGAGCTGTTCACCG | NheI |
| Ku70-GFP-R | GGCACCGGTCTTATCGTCGTCATCCTTGTAATCAG | AgeI |
| Ku70-HA2-F | CCGACCGGTTCAGGGTGGGAGTCATATTACAAAACC | AgeI |
| Ku70-HA2-R | CCGACGCGTCAGTGAACCGAGATTGAGTCAGTGC | MluI |
| GFP-F | GGCAAGCTTACCGGTCGCCACCATGGTGAGCAAGGGCG | HindIII and AgeI |
| GFP-R | CCGCTCGAGCTTATCGTCGTCATCC | XhoI |
| Ku70siR-R | GGCACGCGTGCGGCCGCTTAGTCCTGGAAGTGCTTGGTGAGG | MluI and NotI |
| Ku70Mut6E1-R | GCTCTATTGGAGGAGGTTCGAGAGCCTTCTGGACCAGATTATAAATGC | − |
| Ku70Mut6E2-F | GGTCCAGAAGGCTCTCGAACCTCCTCCAATAGAGCTCTATCGG | − |
| Ku70Mut6E2-R | GGAGGGATGTTCCTCTCGGGTGTGTATCTGCACAATGCTGC | − |
| Ku70Mut6E3-F | GCAGATACACACCCGAGAGGAACATCCCTCCTTATTTTGTGGC | − |
Minus signs indicate that the oligonucleotide does not contain any restriction site used for cloning.