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Table 1.

Parameters describing exponential decay curves generated by nonlinear regression for each posttreatment kinetics

Parameters DMSO DNA-PKi ATMi DNA-PKi + ATMi 
Plateau ± SD (%) 10.71 ± 3.25 50.87 ± 8.79 4.56 ± 6.99 65.21 ± 8.31 
Significance of difference to control − P < 0.01 ns P < 0.001 
Corresponding p-value − 0.0018 0.2393 0.0005 
Significance of difference to DNA-PKi − − P < 0.01 ns 
Corresponding p-value − − 0.002 0.1094 
K ± SD 0.0436 ± 0.0057 0.0254 ± 0.0138 0.0178 ± 0.0036 0.0176 ± 0.0115 
Significance of difference to control − ns P < 0.01 P < 0.05 
Corresponding p-value − 0.103 0.0027 0.0248 
Significance of difference to DNA-PKi − − ns ns 
Corresponding p-value − − 0.4114 0.496 
Half-life (minutes; ln(2)/K) 15.91 27.29 38.83 39.28 
Regression R2 0.9463 0.4856 0.8888 0.4073 
Parameters DMSO DNA-PKi ATMi DNA-PKi + ATMi 
Plateau ± SD (%) 10.71 ± 3.25 50.87 ± 8.79 4.56 ± 6.99 65.21 ± 8.31 
Significance of difference to control − P < 0.01 ns P < 0.001 
Corresponding p-value − 0.0018 0.2393 0.0005 
Significance of difference to DNA-PKi − − P < 0.01 ns 
Corresponding p-value − − 0.002 0.1094 
K ± SD 0.0436 ± 0.0057 0.0254 ± 0.0138 0.0178 ± 0.0036 0.0176 ± 0.0115 
Significance of difference to control − ns P < 0.01 P < 0.05 
Corresponding p-value − 0.103 0.0027 0.0248 
Significance of difference to DNA-PKi − − ns ns 
Corresponding p-value − − 0.4114 0.496 
Half-life (minutes; ln(2)/K) 15.91 27.29 38.83 39.28 
Regression R2 0.9463 0.4856 0.8888 0.4073 

For each condition, the number of Ku foci 5 min after IR was set to 100% and considered as t = 0 min. Nonlinear regressions were then applied using Prism v6.0b to generate curves fitting to a one-phase exponential decay using the formula %RemainingFoci(t) = (100 − Plateau) × exp(−K × t) + Plateau. Minus signs indicate not applicable. K, decay constant; ns, no significant difference.

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