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Table I.

Summary of results for all proteins analyzed in this study

 Notes Lipid bound Localization Ratio FPM/FCyt τ Range (63%) Range (95%) n 
     µm2/s s µm µm  
GFP None None Cytoplasm ND 31.36 ± 5.06 NA NA NA 13 
PM-YFP None Integral PM ND 0.79 ± 0.06 2.12 × 1021 ± 2.11 × 1021 ∞ ∞ 43 
GFP-PH123-MyoX None PIP2 + PIP3 PM 1.15 ± 0.04 0.07 ± 0.02 6.96 ± 0.93 0.98 1.70 14 
PH-PLCδ1-GFP None PIP2 PM 2.07 ± 0.12 1.24 ± 0.14 2.44 ± 0.27 2.46 4.26 52 
PH-PLCδ1R40L-GFP None None Cytoplasm ND 21.47 ± 3.01 NA NA NA 21 
GFP-PH-PH-PLCδ1 None PIP2 PM 3.49 ± 0.17 0.24 ± 0.02 11.13 ± 1.83 2.29 3.97 33 
PH-PH-PLCδ1-GFP None PIP2 PM 4.45 ± 0.40 0.28 ± 0.03 11.08 ± 2.01 2.50 4.33 30 
PHR40L-PH-PLCδ1-GFP None PIP2 PM 2.13 ± 0.12 0.34 ± 0.05 2.87 ± 0.26 1.40 2.43 30 
PH-PLCδ1-GFP CHO-M1 PIP2 PM 1.66 ± 0.11 0.60 ± 0.10 2.21 ± 0.22 1.63 2.82 15 
GFP-PH-PH-PLCδ1 CHO-M1 PIP2 PM 2.99 ± 0.27 0.05 ± 0.01 7.03 ± 0.77 0.85 1.48 13 
GFP-GAP1m LY None Cytoplasm ND 3.96 ± 0.66 NA NA NA 28 
 Insulin PIP3 PM 1.52 ± 0.08 1.33 ± 0.29 3.42 ± 0.44 3.02 5.23 30 
GFP-GAP1IP4BP LY PIP2 PM 2.55 ± 0.11 0.35 ± 0.04 3.50 ± 0.26 1.57 2.72 40 
 Insulin PIP2 + PIP3 PM 4.44 ± 0.27 0.27 ± 0.02 6.46 ± 1.12 1.86 3.22 46 
GFP-GAP1IP4BP 10 µM Wmn None Cytoplasm ND 4.62 ± 0.54 NA NA NA 19 
GFP-GAP1IP4BP-ΔC2 LY PIP2 PM 2.33 ± 0.13 0.41 ± 0.04 3.34 ± 0.50 1.65 2.85 36 
 Insulin PIP2 + PIP3 PM 4.28 ± 0.27 0.49 ± 0.04 7.93 ± 1.78 2.79 4.84 38 
GFP-PH-GAP1IP4BP LY PIP2 PM 3.31 ± 0.18 0.59 ± 0.04 3.64 ± 0.38 2.07 3.58 51 
 Insulin PIP2 + PIP3 PM 4.99 ± 0.37 0.70 ± 0.04 7.37 ± 1.06 3.21 5.56 47 
 Notes Lipid bound Localization Ratio FPM/FCyt τ Range (63%) Range (95%) n 
     µm2/s s µm µm  
GFP None None Cytoplasm ND 31.36 ± 5.06 NA NA NA 13 
PM-YFP None Integral PM ND 0.79 ± 0.06 2.12 × 1021 ± 2.11 × 1021 ∞ ∞ 43 
GFP-PH123-MyoX None PIP2 + PIP3 PM 1.15 ± 0.04 0.07 ± 0.02 6.96 ± 0.93 0.98 1.70 14 
PH-PLCδ1-GFP None PIP2 PM 2.07 ± 0.12 1.24 ± 0.14 2.44 ± 0.27 2.46 4.26 52 
PH-PLCδ1R40L-GFP None None Cytoplasm ND 21.47 ± 3.01 NA NA NA 21 
GFP-PH-PH-PLCδ1 None PIP2 PM 3.49 ± 0.17 0.24 ± 0.02 11.13 ± 1.83 2.29 3.97 33 
PH-PH-PLCδ1-GFP None PIP2 PM 4.45 ± 0.40 0.28 ± 0.03 11.08 ± 2.01 2.50 4.33 30 
PHR40L-PH-PLCδ1-GFP None PIP2 PM 2.13 ± 0.12 0.34 ± 0.05 2.87 ± 0.26 1.40 2.43 30 
PH-PLCδ1-GFP CHO-M1 PIP2 PM 1.66 ± 0.11 0.60 ± 0.10 2.21 ± 0.22 1.63 2.82 15 
GFP-PH-PH-PLCδ1 CHO-M1 PIP2 PM 2.99 ± 0.27 0.05 ± 0.01 7.03 ± 0.77 0.85 1.48 13 
GFP-GAP1m LY None Cytoplasm ND 3.96 ± 0.66 NA NA NA 28 
 Insulin PIP3 PM 1.52 ± 0.08 1.33 ± 0.29 3.42 ± 0.44 3.02 5.23 30 
GFP-GAP1IP4BP LY PIP2 PM 2.55 ± 0.11 0.35 ± 0.04 3.50 ± 0.26 1.57 2.72 40 
 Insulin PIP2 + PIP3 PM 4.44 ± 0.27 0.27 ± 0.02 6.46 ± 1.12 1.86 3.22 46 
GFP-GAP1IP4BP 10 µM Wmn None Cytoplasm ND 4.62 ± 0.54 NA NA NA 19 
GFP-GAP1IP4BP-ΔC2 LY PIP2 PM 2.33 ± 0.13 0.41 ± 0.04 3.34 ± 0.50 1.65 2.85 36 
 Insulin PIP2 + PIP3 PM 4.28 ± 0.27 0.49 ± 0.04 7.93 ± 1.78 2.79 4.84 38 
GFP-PH-GAP1IP4BP LY PIP2 PM 3.31 ± 0.18 0.59 ± 0.04 3.64 ± 0.38 2.07 3.58 51 
 Insulin PIP2 + PIP3 PM 4.99 ± 0.37 0.70 ± 0.04 7.37 ± 1.06 3.21 5.56 47 

All experiments were in HEK cells unless otherwise stated. The localization of the protein (either plasma membrane [PM] or cytoplasm) is noted, along with the lipid bound (PtdIns(4,5)P2 or PtdIns(3,4,5)P3). The parameters are the ratio of fluorescence at the PM verses the cytoplasm (ratio of FPM/FCyt) as a crude index of membrane recruitment, lateral diffusion coefficient (D), and membrane dissociation time constant (τ). The range refers to the expected distance traveled on the membrane for the proportion of these proteins indicated in parentheses, and are estimated as √(2 × D × τ) for 63% and √(2 × D × 3 × τ) for 95%, respectively (Teruel and Meyer, 2000). NA, not applicable; ND, not determined.

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