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Table I.

Effects of wild-type Plk4 and SBM-Plk4 expression on centriole number

Day Cycling cellsa S phase–arrested cellsb 
 Plk4-EGFP Plk4-SBM–EGFP Plk4-EGFP Plk4-SBM–EGFP 
 Total Mean ± SD Median Total Mean ± SD Median Total Mean ± SD Median Total Mean ± SD Median 
100 2.9 ± 3.9 2.0 104 3.5 ± 3.4 2.0 100 3.1 ± 3.1 2.0 100 3.9 ± 4.2 2.0 
112 2.6 ± 3.0 2.0 104 4.3 ± 4.2c 3.0 101 3.5 ± 3.2 2.0 100 4.7 ± 5.3 4.0 
111 3.1 ± 2.7 2.0 103 5.3 ± 4.0c 4.0 100 3.1 ± 3.7 2.0 101 5.1 ± 4.4c 4.0 
113 3.0 ± 2.4 2.0 111 5.3 ± 3.4c 5.0 100 3.4 ± 3.7 2.0 100 6.1 ± 5.0c 5.0 
97 3.2 ± 2.8 2.0 100 7.1 ± 5.8c 6.0 100 2.8 ± 2.7 2.0 100 6.4 ± 5.2c 6.0 
Day Cycling cellsa S phase–arrested cellsb 
 Plk4-EGFP Plk4-SBM–EGFP Plk4-EGFP Plk4-SBM–EGFP 
 Total Mean ± SD Median Total Mean ± SD Median Total Mean ± SD Median Total Mean ± SD Median 
100 2.9 ± 3.9 2.0 104 3.5 ± 3.4 2.0 100 3.1 ± 3.1 2.0 100 3.9 ± 4.2 2.0 
112 2.6 ± 3.0 2.0 104 4.3 ± 4.2c 3.0 101 3.5 ± 3.2 2.0 100 4.7 ± 5.3 4.0 
111 3.1 ± 2.7 2.0 103 5.3 ± 4.0c 4.0 100 3.1 ± 3.7 2.0 101 5.1 ± 4.4c 4.0 
113 3.0 ± 2.4 2.0 111 5.3 ± 3.4c 5.0 100 3.4 ± 3.7 2.0 100 6.1 ± 5.0c 5.0 
97 3.2 ± 2.8 2.0 100 7.1 ± 5.8c 6.0 100 2.8 ± 2.7 2.0 100 6.4 ± 5.2c 6.0 

Expression of Plk4-EGFP and Plk4-SBM–EGFP was driven off of the weak SAS-6p. Positive transfected cells were identified by cotransfection with a plasmid expressing the nuclear protein Nlp-EGFP. Total, total cell number; mean and median columns refer to centriole number.

a

Centrioles were counted beginning 24 h after transfection.

b

24 h after transfection, cells were treated with aphidicolin/HU to arrest them through the time course of this experiment. Centrioles were quantified beginning 48 h after transfection.

c

Indicates a significant difference using an unpaired t test (P < 0.001).

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