S. pombe strains used in this study
| Strain number . | Relevant genotype . | Source . |
|---|---|---|
| MBY102 | ade6-210 ura4-D18 leu1-32 h+ | Laboratory collection |
| MBY297 | mid1-18, leu1-32, ade6-21X, h+ | Laboratory collection |
| MBY461 | cps1-191 sid2-250 | Laboratory collection |
| MBY628 | rlc1-GFP∷ura4+ cdc25-22 h+ | Laboratory collection |
| MBY1148 | cps1-191 ade6-21x ura4-D18 leu1-32 h+ | Laboratory collection |
| MBY1287 | mid1-18 rlc1-GFP∷ura4+ ade6-21x leu1-32 ura4-D18 h− | Laboratory collection |
| MBY1463 | plo1-1, leu1-32, ura4-D18, ade6-21X, h− | Laboratory collection |
| MBY2016 | mid1∷ura4+, leu1-32, ade6-210, h− | Laboratory collection |
| MBY2019 | ade-M216 ura4-D18 leu1-32 dmf1∷ura4+ h- + pAP116 integrated (pmid1-dNES1+2 mid1 leu1+) | Laboratory collection |
| MBY2120 | cps1-191 clp1∷ura4+ | Laboratory collection |
| MBY2378 | cdc25-22 rlc-GFP∷ura4+ mid1∷ura4+ | Laboratory collection |
| MBY2541 | pCDL514 (pREP1-mad2) in MBY1287 | Laboratory collection |
| MBY2591 | cps1-191 rlc1-GFP∷ura4+ ura4-D18 leu1-32 ade6-M21x h+ | Laboratory collection |
| MBY2603 | cdc16-116 uch2-GFP∷ura4+ rlc-GFP∷leu1+ | This study |
| MBY2697 | cps1-191 mid1-ΔNES1+2 leu+ | This study |
| MBY3067 | mid1-18 clp1∷ura4+ rlc1-GFP∷ura4+ ura4-D18 leu1-32 ade6-21x | Laboratory collection |
| MBY3503 | cdc16-116 mid1-18 uch2-GFP∷ura4+ rlc1-GFP∷leu1+ | This study |
| MBY3504 | cdc16-116 mid1∷ura4 uch2-GFP∷ura4+ rlc1-GFP∷leu1+ | This study |
| MBY3957 | mid1-18 cps1-191 rlc1-GFP∷ura4+ h+ | Laboratory collection |
| MBY4123 | cps1-191 mid1∷ura4+ rlc1-GFP h+ | This study |
| MBY5242 | cps1-191 plo1-1 rlc1-3GFP | This study |
| MBY5727 | cps1-191 mid1∷ura4 rlc1-GFP∷ura+ pcp1-GFP∷KanMX6 | This study |
| MBY5730 | cps1-191 rlc1-GFP∷ura+ pcp1-GFP∷KanMX6 | This study |
| MBY5737 | cdc25-22 cdc12-GFP∷ura4+ | This study |
| MBY5738 | cdc25-22 mid1∷ura4+ cdc12-GFP∷ura4+ | This study |
| MBY5739 | cdc25-22 cdc15-GFP∷KanMX6 | This study |
| MBY5740 | cdc25-22 mid1∷ura4+ cdc15-GFP∷KanMX6 | This study |
| Strain number . | Relevant genotype . | Source . |
|---|---|---|
| MBY102 | ade6-210 ura4-D18 leu1-32 h+ | Laboratory collection |
| MBY297 | mid1-18, leu1-32, ade6-21X, h+ | Laboratory collection |
| MBY461 | cps1-191 sid2-250 | Laboratory collection |
| MBY628 | rlc1-GFP∷ura4+ cdc25-22 h+ | Laboratory collection |
| MBY1148 | cps1-191 ade6-21x ura4-D18 leu1-32 h+ | Laboratory collection |
| MBY1287 | mid1-18 rlc1-GFP∷ura4+ ade6-21x leu1-32 ura4-D18 h− | Laboratory collection |
| MBY1463 | plo1-1, leu1-32, ura4-D18, ade6-21X, h− | Laboratory collection |
| MBY2016 | mid1∷ura4+, leu1-32, ade6-210, h− | Laboratory collection |
| MBY2019 | ade-M216 ura4-D18 leu1-32 dmf1∷ura4+ h- + pAP116 integrated (pmid1-dNES1+2 mid1 leu1+) | Laboratory collection |
| MBY2120 | cps1-191 clp1∷ura4+ | Laboratory collection |
| MBY2378 | cdc25-22 rlc-GFP∷ura4+ mid1∷ura4+ | Laboratory collection |
| MBY2541 | pCDL514 (pREP1-mad2) in MBY1287 | Laboratory collection |
| MBY2591 | cps1-191 rlc1-GFP∷ura4+ ura4-D18 leu1-32 ade6-M21x h+ | Laboratory collection |
| MBY2603 | cdc16-116 uch2-GFP∷ura4+ rlc-GFP∷leu1+ | This study |
| MBY2697 | cps1-191 mid1-ΔNES1+2 leu+ | This study |
| MBY3067 | mid1-18 clp1∷ura4+ rlc1-GFP∷ura4+ ura4-D18 leu1-32 ade6-21x | Laboratory collection |
| MBY3503 | cdc16-116 mid1-18 uch2-GFP∷ura4+ rlc1-GFP∷leu1+ | This study |
| MBY3504 | cdc16-116 mid1∷ura4 uch2-GFP∷ura4+ rlc1-GFP∷leu1+ | This study |
| MBY3957 | mid1-18 cps1-191 rlc1-GFP∷ura4+ h+ | Laboratory collection |
| MBY4123 | cps1-191 mid1∷ura4+ rlc1-GFP h+ | This study |
| MBY5242 | cps1-191 plo1-1 rlc1-3GFP | This study |
| MBY5727 | cps1-191 mid1∷ura4 rlc1-GFP∷ura+ pcp1-GFP∷KanMX6 | This study |
| MBY5730 | cps1-191 rlc1-GFP∷ura+ pcp1-GFP∷KanMX6 | This study |
| MBY5737 | cdc25-22 cdc12-GFP∷ura4+ | This study |
| MBY5738 | cdc25-22 mid1∷ura4+ cdc12-GFP∷ura4+ | This study |
| MBY5739 | cdc25-22 cdc15-GFP∷KanMX6 | This study |
| MBY5740 | cdc25-22 mid1∷ura4+ cdc15-GFP∷KanMX6 | This study |