Human oligonucleotide primers used for RT-PCR
| Gene . | Primer Sequence . | Strand . | Product size . | Temperature . | PCR cycle . | Reference . |
|---|---|---|---|---|---|---|
| bp | °C | |||||
| SIK | 5′-GTCTGGGCGCACCTTTAGCA-3′ | + | 387 | 62 | 27 | NM_173354 |
| 5′-CAGCCTTTCGCCCAGTCTTT-3′ | − | |||||
| IDB2 | 5′-ACGACCCGATGAGCCTGCTA-3′ | + | 213 | 55 | 24 | Kowanetz et al. (2004) |
| 5′-TCCTGGAGCGCTGGTTCTG-3′ | − | |||||
| CDKN1A (p21) | 5′-CTGCCCAAGCTCTACCTTCC-3′ | + | 123 | 57 | 28 | Kowanetz et al. (2004) |
| 5′-CAGGTCCACATGGTCTTCCT-3′ | − | |||||
| c-MYC | 5′-ACCCGGACGACGAGACCTTCATCA-3′ | + | 683 | 63 | 23 | Kowanetz et al. (2004) |
| 5′-GGGGCTGGTGCATTTTCGGTTGTT-3′ | − | |||||
| MADH7 (Smad7) | 5′-CAGATTCCCAACTTCTTCTG-3′ | + | 533 | 53 | 27 | Kowanetz et al. (2004) |
| 5′-GTTGAAGATGACCTCCAGCC-3′ | − | |||||
| GAPDH | 5′-ATCACTGCCACCCAGAAGAC-3′ | + | 443 | 57 | 22 | Kowanetz et al. (2004) |
| 5′-ATGAGGTCCACCACCCTGTT-3′ | − |
| Gene . | Primer Sequence . | Strand . | Product size . | Temperature . | PCR cycle . | Reference . |
|---|---|---|---|---|---|---|
| bp | °C | |||||
| SIK | 5′-GTCTGGGCGCACCTTTAGCA-3′ | + | 387 | 62 | 27 | NM_173354 |
| 5′-CAGCCTTTCGCCCAGTCTTT-3′ | − | |||||
| IDB2 | 5′-ACGACCCGATGAGCCTGCTA-3′ | + | 213 | 55 | 24 | Kowanetz et al. (2004) |
| 5′-TCCTGGAGCGCTGGTTCTG-3′ | − | |||||
| CDKN1A (p21) | 5′-CTGCCCAAGCTCTACCTTCC-3′ | + | 123 | 57 | 28 | Kowanetz et al. (2004) |
| 5′-CAGGTCCACATGGTCTTCCT-3′ | − | |||||
| c-MYC | 5′-ACCCGGACGACGAGACCTTCATCA-3′ | + | 683 | 63 | 23 | Kowanetz et al. (2004) |
| 5′-GGGGCTGGTGCATTTTCGGTTGTT-3′ | − | |||||
| MADH7 (Smad7) | 5′-CAGATTCCCAACTTCTTCTG-3′ | + | 533 | 53 | 27 | Kowanetz et al. (2004) |
| 5′-GTTGAAGATGACCTCCAGCC-3′ | − | |||||
| GAPDH | 5′-ATCACTGCCACCCAGAAGAC-3′ | + | 443 | 57 | 22 | Kowanetz et al. (2004) |
| 5′-ATGAGGTCCACCACCCTGTT-3′ | − |
A previously published reference or the NCBI database accession number is indicated.