Skip to Main Content
Table I.

Duration of mitosis in RPE-1 cells exposed to various drugs that perturb spindle formation (37°C)a

Drug/concentration
MTs
Duration of mitosis ± SD (N)b
Approximate hours in mitosisc
Survival
    %d 
Control 18 ± 3 (33) 0.3 100 
3.2 μM nocodazole − 1,140 ± 732 (25) 19 71 
250 nM colcemid − 1,303 ± 362 (24) 22 60 
25 nM vinblastine − 1,784 ± 602 (24)e 30 26 
1 μM vinblastine − 1,820 ± 530 (37) 30 21 
1 μM maytansine − 1,558 ± 407 (50) 26 50 
500 nM taxol 744 ± 399 (117) 12 81 
5.0 μM S-Trityl-l-cysteinef 1,179 ± 310 (160) 20 84 
2.5 μM dimethylenastrong 1,218 ± 424 (241) 20 80 
50 nM nocodazole 153 ± 58 (200) 100 
5 nM vinblastine 234 ± 140 (180) 95 
5 nM taxol 161 ± 85 (171) 99 
Drug/concentration
MTs
Duration of mitosis ± SD (N)b
Approximate hours in mitosisc
Survival
    %d 
Control 18 ± 3 (33) 0.3 100 
3.2 μM nocodazole − 1,140 ± 732 (25) 19 71 
250 nM colcemid − 1,303 ± 362 (24) 22 60 
25 nM vinblastine − 1,784 ± 602 (24)e 30 26 
1 μM vinblastine − 1,820 ± 530 (37) 30 21 
1 μM maytansine − 1,558 ± 407 (50) 26 50 
500 nM taxol 744 ± 399 (117) 12 81 
5.0 μM S-Trityl-l-cysteinef 1,179 ± 310 (160) 20 84 
2.5 μM dimethylenastrong 1,218 ± 424 (241) 20 80 
50 nM nocodazole 153 ± 58 (200) 100 
5 nM vinblastine 234 ± 140 (180) 95 
5 nM taxol 161 ± 85 (171) 99 
a

Minutes from first frame after NEB to first frame including the initial signs of cytokinesis and/or telophase membrane blebbing (in cells that exited mitosis).

b

N = total numbers of cells (pooled from ≥2 separate long-term recordings obtained on different days).

c

Mean duration of mitosis in hours.

d

Percentage of cells that exited mitosis (versus dying in mitosis).

e

These data are from a single experiment.

f

An Eg5 inhibitor (and nonnatural derivative of the α–amino acid cysteine).

g

An Eg5 inhibitor (a new monastrol analogue).

Close Modal

or Create an Account

Close Modal
Close Modal