DT40 strains used in this study
| Genotype . | Disrupted gene (selective marker) . | Expression plasmid . | Reference . |
|---|---|---|---|
| blm | BLM (His/Bsr) | W. Wang et al., 2000 | |
| wrn | WRN (His/Bsr) | Imamura et al., 2002 | |
| rad52 | RAD52 (His/Bsr) | Yamaguchi-Iwai et al., 1998 | |
| ku70 | KU70 (His/Bsr) | Takata et al., 1998 | |
| rad18 | RAD18 (His/Hyg) | Yamashita et al., 2002 | |
| top3α | TOP3α (Neo/His) | FLAG-mTOP3α: Hyg | Seki et al., 2006a |
| top3β | TOP3β (Puro/Bsr) | Seki et al., 2006a | |
| atm | ATM (Neo/Puro) | Takao et al., 1999 | |
| rad52/blm | RAD52 (His/Bsr), BLM (Hyg/Puro) | This study | |
| ku70/blm | KU70 (His/Bsr), BLM (Hyg/Neo) | This study | |
| rad18/blm | RAD18 (His/Hyg), BLM (Bsr/Neo) | This study | |
| top3α/blm | TOP3α (Neo/His), BLM (Puro/Bsr) | FLAG-mTOP3α: Hyg | Seki et al., 2006a |
| top3β/blm | TOP3β (Puro/Bsr), BLM (Neo/His) | Seki et al., 2006a | |
| xrcc3+hXRCC3 | XRCC3 (His/Bsr) | hXRCC3: Neo | Ishiai et al., 2004 |
| xrcc3 | XRCC3 (His/Bsr) | Ishiai et al., 2004 | |
| xrcc3/blm+hXRCC3 | XRCC3 (His/Bsr), BLM (Eco/Puro) | hXRCC3: Neo | This study |
| xrcc3/blm | XRCC3 (His/Bsr), BLM (Eco/Puro) | This study | |
| xrcc3/wrn+hXRCC3 | XRCC3 (His/Bsr), WRN (Eco/Puro) | hXRCC3: Neo | This study |
| xrcc3/wrn | XRCC3 (His/Bsr), WRN (Eco/Puro) | This study | |
| xrcc3/wrn/blm+hXRCC3 | XRCC3 (His/Bsr), WRN (Eco/Puro), BLM (Bleo/Hyg) | hXRCC3: Neo | This study |
| xrcc3/wrn/blm | XRCC3 (His/Bsr), WRN (Eco/Puro), BLM (Bleo/Hyg) | This study | |
| xrcc3/rad54+hXRCC3 | XRCC3 (His/Bsr), RAD54 (Hyg/Puro) | hXRCC3: Neo | This study |
| xrcc3/rad54 | XRCC3 (His/Bsr), RAD54 (Hyg/Puro) | This study | |
| xrcc3/blm/rad54+hXRCC3 | XRCC3 (His/Bsr), BLM (Eco/Puro), RAD54 (Hyg/Bleo) | hXRCC3: Neo | This study |
| xrcc3/blm/rad54 | XRCC3 (His/Bsr), BLM (Eco/Puro), RAD54 (Hyg/Bleo) | This study |
| Genotype . | Disrupted gene (selective marker) . | Expression plasmid . | Reference . |
|---|---|---|---|
| blm | BLM (His/Bsr) | W. Wang et al., 2000 | |
| wrn | WRN (His/Bsr) | Imamura et al., 2002 | |
| rad52 | RAD52 (His/Bsr) | Yamaguchi-Iwai et al., 1998 | |
| ku70 | KU70 (His/Bsr) | Takata et al., 1998 | |
| rad18 | RAD18 (His/Hyg) | Yamashita et al., 2002 | |
| top3α | TOP3α (Neo/His) | FLAG-mTOP3α: Hyg | Seki et al., 2006a |
| top3β | TOP3β (Puro/Bsr) | Seki et al., 2006a | |
| atm | ATM (Neo/Puro) | Takao et al., 1999 | |
| rad52/blm | RAD52 (His/Bsr), BLM (Hyg/Puro) | This study | |
| ku70/blm | KU70 (His/Bsr), BLM (Hyg/Neo) | This study | |
| rad18/blm | RAD18 (His/Hyg), BLM (Bsr/Neo) | This study | |
| top3α/blm | TOP3α (Neo/His), BLM (Puro/Bsr) | FLAG-mTOP3α: Hyg | Seki et al., 2006a |
| top3β/blm | TOP3β (Puro/Bsr), BLM (Neo/His) | Seki et al., 2006a | |
| xrcc3+hXRCC3 | XRCC3 (His/Bsr) | hXRCC3: Neo | Ishiai et al., 2004 |
| xrcc3 | XRCC3 (His/Bsr) | Ishiai et al., 2004 | |
| xrcc3/blm+hXRCC3 | XRCC3 (His/Bsr), BLM (Eco/Puro) | hXRCC3: Neo | This study |
| xrcc3/blm | XRCC3 (His/Bsr), BLM (Eco/Puro) | This study | |
| xrcc3/wrn+hXRCC3 | XRCC3 (His/Bsr), WRN (Eco/Puro) | hXRCC3: Neo | This study |
| xrcc3/wrn | XRCC3 (His/Bsr), WRN (Eco/Puro) | This study | |
| xrcc3/wrn/blm+hXRCC3 | XRCC3 (His/Bsr), WRN (Eco/Puro), BLM (Bleo/Hyg) | hXRCC3: Neo | This study |
| xrcc3/wrn/blm | XRCC3 (His/Bsr), WRN (Eco/Puro), BLM (Bleo/Hyg) | This study | |
| xrcc3/rad54+hXRCC3 | XRCC3 (His/Bsr), RAD54 (Hyg/Puro) | hXRCC3: Neo | This study |
| xrcc3/rad54 | XRCC3 (His/Bsr), RAD54 (Hyg/Puro) | This study | |
| xrcc3/blm/rad54+hXRCC3 | XRCC3 (His/Bsr), BLM (Eco/Puro), RAD54 (Hyg/Bleo) | hXRCC3: Neo | This study |
| xrcc3/blm/rad54 | XRCC3 (His/Bsr), BLM (Eco/Puro), RAD54 (Hyg/Bleo) | This study |
Bleo, bleomycin; Bsr, blasticidin; Eco, ecogpt; His, histidinol; Hyg, hygromycin; Neo, neomycin; Puro, puromycin.