Rockefeller University Press

Table I.

Primers and cDNA products for construction of Pax7-deletion mutants

cDNA	Forward primer (5′→3′)/target location (nt)	Reverse primer (5′→3′)/target location (nt)
FL	GGATCCATGGCGGCCCTTCCC/1–15	CTCGAGCTAGTAGGCTTGTCCCGTTTCCAC/1489–1512
ΔN	GGATCCGGGAAGAAAGAGGACGACGAG/487–507	CTCGAGCTAGTAGGCTTGTCCCGTTTCCAC/1489–1512
ΔC	GGATCCATGGCGGCCCTTCCC/1–15	CTCGAGCTATCCTGCCTGCTTGCGCCA/811–828
ΔHD
N-Pax7^{^a}	GGATCCATGGCGGCCCTTCCC/1–15	CTGGTTAGCGCGCTGCTTGCGCTTCAG/631–648
C-Pax7^{^b}	AAGCAGCGCGCTAACCAGCTGGCCGCC/826–843	CTCGAGCTAGTAGGCTTGTCCCGTTTCCAC/1489–1512
ΔHD^{^c}	GGATCCATGGCGGCCCTTCCC/1–15	CTCGAGCTAGTAGGCTTGTCCCGTTTCCAC/1489–1512
ΔNO	GGATCCCAGCGCCGCAGTCGGACC/643–660	CTCGAGCTAGTAGGCTTGTCCCGTTTCCAC/1489–1512
ΔNHD	GGATCCGCTAACCAGCTGGCCGCC/826–843	CTCGAGCTAGTAGGCTTGTCCCGTTTCCAC/1489–1512

cDNA	Forward primer (5′→3′)/target location (nt)	Reverse primer (5′→3′)/target location (nt)
FL	GGATCCATGGCGGCCCTTCCC/1–15	CTCGAGCTAGTAGGCTTGTCCCGTTTCCAC/1489–1512
ΔN	GGATCCGGGAAGAAAGAGGACGACGAG/487–507	CTCGAGCTAGTAGGCTTGTCCCGTTTCCAC/1489–1512
ΔC	GGATCCATGGCGGCCCTTCCC/1–15	CTCGAGCTATCCTGCCTGCTTGCGCCA/811–828
ΔHD
N-Pax7^{^a}	GGATCCATGGCGGCCCTTCCC/1–15	CTGGTTAGCGCGCTGCTTGCGCTTCAG/631–648
C-Pax7^{^b}	AAGCAGCGCGCTAACCAGCTGGCCGCC/826–843	CTCGAGCTAGTAGGCTTGTCCCGTTTCCAC/1489–1512
ΔHD^{^c}	GGATCCATGGCGGCCCTTCCC/1–15	CTCGAGCTAGTAGGCTTGTCCCGTTTCCAC/1489–1512
ΔNO	GGATCCCAGCGCCGCAGTCGGACC/643–660	CTCGAGCTAGTAGGCTTGTCCCGTTTCCAC/1489–1512
ΔNHD	GGATCCGCTAACCAGCTGGCCGCC/826–843	CTCGAGCTAGTAGGCTTGTCCCGTTTCCAC/1489–1512

GGATCC, BamHI recognition site; CTCGAG, XhoI recognition site.

N terminus/paired box/octapeptide.

Transactivation domain.

N-Pax7/C-Pax7 template.