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Table I.

Velocities of retrograde actin cable flow in myosin mutants and the corresponding wild-type strains

Strain
Genotype
Retrograde actin cable flow rate
23°C
37°C
  μm/s μm/s 
S288C wild type for myo1Δ and
     myo2-66 myo4Δ (YCY027) MATα his3Δ leu2Δ met15Δ ura3Δ ABP140∷GFP∷KanMX6 0.38 ± 0.08 0.38 ± 0.08 
W303 wild type for myo3Δ myo5Δ
     (YCY006) MATα can1-100 ade2-1 his3-11 leu2-3,112 ura3-1 trp1-1
     ABP140GFPKanMX6 0.36 ± 0.1 nd 
myo1Δ (THY120) MATα leu2-3,112 ura3-52 ade1-101 myo1ΔURA3
     ABP140∷GFP∷KanMX6 0.19 ± 0.07 nd 
myo2-66 myo4Δ (VSY21) MATα leu2-3,112 his3-200 ura3-52 myo2-66∷HIS3
     myo4ΔURA3 0.40 ± 0.06 0.38 ± 0.08 
myo3Δmyo5Δ (YCY009) MATα can1-100 ade2-1 his3-11 leu2-3,112 ura3-1 trp1-1
     myo3Δ∷HIS3 myo5ΔTRP1 ABP140GFPKanMX6 0.29 ± 0.06 nd 
Strain
Genotype
Retrograde actin cable flow rate
23°C
37°C
  μm/s μm/s 
S288C wild type for myo1Δ and
     myo2-66 myo4Δ (YCY027) MATα his3Δ leu2Δ met15Δ ura3Δ ABP140∷GFP∷KanMX6 0.38 ± 0.08 0.38 ± 0.08 
W303 wild type for myo3Δ myo5Δ
     (YCY006) MATα can1-100 ade2-1 his3-11 leu2-3,112 ura3-1 trp1-1
     ABP140GFPKanMX6 0.36 ± 0.1 nd 
myo1Δ (THY120) MATα leu2-3,112 ura3-52 ade1-101 myo1ΔURA3
     ABP140∷GFP∷KanMX6 0.19 ± 0.07 nd 
myo2-66 myo4Δ (VSY21) MATα leu2-3,112 his3-200 ura3-52 myo2-66∷HIS3
     myo4ΔURA3 0.40 ± 0.06 0.38 ± 0.08 
myo3Δmyo5Δ (YCY009) MATα can1-100 ade2-1 his3-11 leu2-3,112 ura3-1 trp1-1
     myo3Δ∷HIS3 myo5ΔTRP1 ABP140GFPKanMX6 0.29 ± 0.06 nd 

nd, no data.

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