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Table I.

Shedding of EBA-175 at erythrocyte invasion occurs to the same extent whether or not it is used as the dominant invasion ligand


Proportion of rings reactive with anti–region VI
 antibodies in IFAa
P. falciparum line
Erythrocyte pretreatment
untreated
mock
neuraminidaseb
 % % % 
W2mef 13.4 ± 3.8 NDc NDc 
3D7 16.7 ± 3.5 14.9 ± 3.2 15.4 ± 5.1d 

Proportion of rings reactive with anti–region VI
 antibodies in IFAa
P. falciparum line
Erythrocyte pretreatment
untreated
mock
neuraminidaseb
 % % % 
W2mef 13.4 ± 3.8 NDc NDc 
3D7 16.7 ± 3.5 14.9 ± 3.2 15.4 ± 5.1d 
a

Figures shown are derived from three experiments. At least 1,000 rings were examined in each case. 95% confidence levels are indicated.

b

Erythrocytes were neuraminidase treated or mock treated as described in Materials and methods. The efficiency of the treatment was assessed using an overlay assay to confirm lack of EBA-175 binding (Fig. S1).

c

Invasion of neuraminidase-treated erythrocytes by W2mef was very inefficient (≤2.0% of invasion into mock-treated cells) as expected, so these rings were not examined by IFA.

d

Invasion of neuraminidase-treated erythrocytes by 3D7 was 89% of invasion into mock-treated cells, in agreement with previous findings (Duraisingh et al., 2003).

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