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Table II.

Plasmids

Plasmid
Description
Reference
pAZ002 ASI1-3xHA in pRS316 This study 
pAZ011 asi1-21-3xHA in pRS316 This study 
pAZ012 asi1-22-3xHA in pRS316 This study 
pAZ014 ASI1-3xHA in pRS202 This study 
pAZ015 asi1-21-3xHA in pRS202 This study 
pAZ016 asi1-22-3xHA in pRS202 This study 
pAZ020 ASI1-3xHA-CHO in pRS316 This study 
pAZ024 asi1Δ8∷kanMX in pRS316 This study 
pAZ025/26/28/30/33 BglII sites introduced into ASI1 in pAZ020 This study 
pAZ034/35/37/39/73 suc2 topology reporter inserted into BglII sites in
     pAZ025/26/28/30/33 This study 
pCA030 PAGP1-lacZ in pRS317 Andréasson and Ljungdahl, 2004  
pCA047 STP1-3xHA in pRS316 Andréasson and Ljungdahl, 2002  
pCA078 PHXT7-STP1-3xHA in pRS202 Andréasson and Ljungdahl, 2002  
pCA088 STP1-3xHA in pRS316 This work 
pCA111 STP2-3xHA in pRS316 Andréasson and Ljungdahl, 2002  
pCA145 lexA in pRS202 This study 
pCA153 lexA in 2 μm LYS2 This study 
pCA160 2 μm LYS2 PADH1 ΔSph1 Andréasson and Ljungdahl, 2004  
pCA181 STP1-6xHA in pRS316 This study 
pCA227 PGNP1-lacZ in CEN URA3 This study 
pMH4 ASI1 in pRS316 Forsberg et al., 2001  
pMB09 STP1-13xMYC in pRS316 This study 
pMB10 13xMYC-STP1-6xHA in pRS316 This study 
pMB12 STP2-3xHA in pRS202 This study 
pMB13 STP2-6xHA in pRS202 This study 
pMB16 lexA-Stp1(aa 2–69) in 2 μm LYS2 This study 
pMB17 lexA-Stp2(aa 2–77) in 2 μm LYS2 This study 
pMB18 PGAL1-OPlexA-lacZ in CEN URA3 This study 
pMB27 STP2-13xMYC in pRS316 This study 
pMB30 13xMYC-STP2-6xHA in pRS202 This study 
pRS202 2 μm URA3 Connelly and Hieter, 1996  
pRS316 CEN URA3 Sikorski and Hieter, 1989  
pRS317 CEN LYS2 Sikorski and Hieter, 1989  
YCpAGP1-LacZ PAGP1-lacZ in CEN URA3 Iraqui et al., 1999  
Plasmid
Description
Reference
pAZ002 ASI1-3xHA in pRS316 This study 
pAZ011 asi1-21-3xHA in pRS316 This study 
pAZ012 asi1-22-3xHA in pRS316 This study 
pAZ014 ASI1-3xHA in pRS202 This study 
pAZ015 asi1-21-3xHA in pRS202 This study 
pAZ016 asi1-22-3xHA in pRS202 This study 
pAZ020 ASI1-3xHA-CHO in pRS316 This study 
pAZ024 asi1Δ8∷kanMX in pRS316 This study 
pAZ025/26/28/30/33 BglII sites introduced into ASI1 in pAZ020 This study 
pAZ034/35/37/39/73 suc2 topology reporter inserted into BglII sites in
     pAZ025/26/28/30/33 This study 
pCA030 PAGP1-lacZ in pRS317 Andréasson and Ljungdahl, 2004  
pCA047 STP1-3xHA in pRS316 Andréasson and Ljungdahl, 2002  
pCA078 PHXT7-STP1-3xHA in pRS202 Andréasson and Ljungdahl, 2002  
pCA088 STP1-3xHA in pRS316 This work 
pCA111 STP2-3xHA in pRS316 Andréasson and Ljungdahl, 2002  
pCA145 lexA in pRS202 This study 
pCA153 lexA in 2 μm LYS2 This study 
pCA160 2 μm LYS2 PADH1 ΔSph1 Andréasson and Ljungdahl, 2004  
pCA181 STP1-6xHA in pRS316 This study 
pCA227 PGNP1-lacZ in CEN URA3 This study 
pMH4 ASI1 in pRS316 Forsberg et al., 2001  
pMB09 STP1-13xMYC in pRS316 This study 
pMB10 13xMYC-STP1-6xHA in pRS316 This study 
pMB12 STP2-3xHA in pRS202 This study 
pMB13 STP2-6xHA in pRS202 This study 
pMB16 lexA-Stp1(aa 2–69) in 2 μm LYS2 This study 
pMB17 lexA-Stp2(aa 2–77) in 2 μm LYS2 This study 
pMB18 PGAL1-OPlexA-lacZ in CEN URA3 This study 
pMB27 STP2-13xMYC in pRS316 This study 
pMB30 13xMYC-STP2-6xHA in pRS202 This study 
pRS202 2 μm URA3 Connelly and Hieter, 1996  
pRS316 CEN URA3 Sikorski and Hieter, 1989  
pRS317 CEN LYS2 Sikorski and Hieter, 1989  
YCpAGP1-LacZ PAGP1-lacZ in CEN URA3 Iraqui et al., 1999  
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