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Table III.

Accumulation of centrosomal components in mitotic cells after cosilencing of Dgrip75, Dgrip128, Dgrip163, and Dgp71WD in cultured cells


Staining

Control

Cosilencing


Bipolar
Monopolar
 % % % 
γ-Tubulin 100 (n = 22) 100 (n = 88) 100 (n = 102) 
Dgrip84 98 ± 4a 99 ± 3 100 (n = 76) 
Dgrip91 98 ± 4 100 (n = 112) 98 ± 4 
Dgrip75 90 ± 6 14 ± 6 0 (n = 70) 
Dgrip128 88 ± 6 2 ± 2 0 (n = 62) 
Dgrip163 94 ± 5 0 (n = 61) 0 (n = 50) 
Dgp71WD
 
95 ± 2
 
0 (n = 45)
 
4 ± 6
 

Staining

Control

Cosilencing


Bipolar
Monopolar
 % % % 
γ-Tubulin 100 (n = 22) 100 (n = 88) 100 (n = 102) 
Dgrip84 98 ± 4a 99 ± 3 100 (n = 76) 
Dgrip91 98 ± 4 100 (n = 112) 98 ± 4 
Dgrip75 90 ± 6 14 ± 6 0 (n = 70) 
Dgrip128 88 ± 6 2 ± 2 0 (n = 62) 
Dgrip163 94 ± 5 0 (n = 61) 0 (n = 50) 
Dgp71WD
 
95 ± 2
 
0 (n = 45)
 
4 ± 6
 

Microtubules were stained with antibodies against α-tubulin and chromosomes with DAPI, whereas the spindle poles were immunostained with antibodies against γ-tubulin, Dgrip84, Dgrip91, Dgrip75, Dgrip128, Dgrip163, and Dgp71WD. For a correct interpretation, we need to keep in mind a decrease in the total amount of γTuRC–specific components in-depleted cells. n, number of prometaphases/metaphases analyzed.

a

Confidence intervals calculated for P95.

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