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Table II.

Distribution of the different mitotic stages after Dgrip75 depletion in cultured cells


Mitotic figures

Control
 n = 100

Dgrip75RNAin = 244
 
Unfocuseda 21 ± 8b 24 ± 6 
Prometaphases/metaphases   
    Symmetrical bipolar 28 ± 9 20 ± 5 
    Abnormal bipolar 4 ± 4 22 ± 6 
    Monopolar 16 ± 4 
Postmetaphase stages
 
47 ± 10
 
18 ± 4
 

Mitotic figures

Control
 n = 100

Dgrip75RNAin = 244
 
Unfocuseda 21 ± 8b 24 ± 6 
Prometaphases/metaphases   
    Symmetrical bipolar 28 ± 9 20 ± 5 
    Abnormal bipolar 4 ± 4 22 ± 6 
    Monopolar 16 ± 4 
Postmetaphase stages
 
47 ± 10
 
18 ± 4
 

Mitotic stages were determined after chromosome staining (DAPI) and immunolabeling of microtubules (α-tubulin antibodies). n, number of mitotic spindles analyzed. These results are representative of three independent experiments.

a

Spindles with no evident polarity, which represent mainly prophase stages or highly abnormal spindles.

b

Confidence intervals calculated for P95.

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