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Table I.

Accumulation of centrosomal components in mitotic cells after Dgrip75 depletion in cultured cells


Staining

Control

Dgrip75RNAi


Bipolar
Monopolar
 % % % 
γ-Tubulin 100 (n = 100) 100 (n = 124) 95 (n = 66) 
Dgrip84 99 (n = 186) 98 (n = 170) 98 (n = 50) 
Dgrip91 91 (n = 103) 100 (n = 55) 100 (n = 48) 
Dgrip75 100 (n = 95) 6 (n = 70) 0 (n = 52) 
Dgrip128 78 (n = 71) 3 (n = 93) nd 
Dgrip163 98 (n = 132) 24 (n = 119) nd 
Dgp71WD
 
95 (n = 109)
 
100 (n = 65)
 
100 (n = 43)
 

Staining

Control

Dgrip75RNAi


Bipolar
Monopolar
 % % % 
γ-Tubulin 100 (n = 100) 100 (n = 124) 95 (n = 66) 
Dgrip84 99 (n = 186) 98 (n = 170) 98 (n = 50) 
Dgrip91 91 (n = 103) 100 (n = 55) 100 (n = 48) 
Dgrip75 100 (n = 95) 6 (n = 70) 0 (n = 52) 
Dgrip128 78 (n = 71) 3 (n = 93) nd 
Dgrip163 98 (n = 132) 24 (n = 119) nd 
Dgp71WD
 
95 (n = 109)
 
100 (n = 65)
 
100 (n = 43)
 

Microtubules were stained with antibodies against α-tubulin and chromosomes with DAPI, whereas the spindle poles were immunostained with antibodies against γ-tubulin, Dgrip84, Dgrip91, Dgrip75, Dgrip128, Dgrip163, and Dgp71WD. For a correct interpretation, we need to keep in mind a decrease in the total amount of Dgrip128 and Dgrip163 in Dgrip75-depleted cells. n, number of prometaphases/metaphases analyzed. nd, not determined.

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