Vascular morphogenesis in P-Sp explants
. | Frequency . | . | |
|---|---|---|---|
| Phenotype
Tstarta → Tobserved . | Controlbn (%) . | Mutant
n (%) . | |
| Sprout → growthc | 58 (56.3) | 40 (35.1) | |
| Sprout → no change | 14 (13.6) | 15 (13.2) | |
| Sprout → regressiond | 31 (30.1) | 59 (51.8) | |
| Subtotal | 103 (100) | 114 (100) | |
| Network → growthe | 39 (45.9) | 22 (23.9) | |
| Network → no change | 30 (35.3) | 23 (25.0) | |
| Network → regressiond | 16 (18.8) | 47 (51.1) | |
| Subtotal | 85 (100) | 92 (100) | |
. | Frequency . | . | |
|---|---|---|---|
| Phenotype
Tstarta → Tobserved . | Controlbn (%) . | Mutant
n (%) . | |
| Sprout → growthc | 58 (56.3) | 40 (35.1) | |
| Sprout → no change | 14 (13.6) | 15 (13.2) | |
| Sprout → regressiond | 31 (30.1) | 59 (51.8) | |
| Subtotal | 103 (100) | 114 (100) | |
| Network → growthe | 39 (45.9) | 22 (23.9) | |
| Network → no change | 30 (35.3) | 23 (25.0) | |
| Network → regressiond | 16 (18.8) | 47 (51.1) | |
| Subtotal | 85 (100) | 92 (100) | |
The subcategories were defined as the phenotype at the initial point of time-lapse videos (Tstart). The videos were captured at 10-min intervals for 24–48 h.
Controls: Tie2-Cre;fakflox/+ or fakflox/+; mutants: Tie2-Cre;fakflox/flox.
Elongation, widening, and network formation from sprouts.
Reduction in length or size and degeneration, including cell death.
Increased network complexity and caliber.