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Table II.

The GPR3-dependent activation of Gs in the oocyte is independent of follicle cells



PM/CYTO

PM/CYTO

PM/CYTO (Gpr3/)
Specimen
 
(Gpr3+/+)
 
(Gpr3/)
 
PM/CYTO (Gpr3+/+)
 
Follicle-enclosed
   oocytesd 3.1 ± 0.2
 (n = 22)a 7.1 ± 0.4
 (n = 30)b 2.3 ± 0.2c 
Isolated oocytese 8.8 ± 0.8
 (n = 19) 21.8 ± 1.2
 (n = 18) 2.5 ± 0.3c 
Isolated oocytes  (with optical
   correction)
 
4.1 ± 0.5
 (n = 19)a
 
10.1 ± 1.0
 (n = 18)b
 

 


PM/CYTO

PM/CYTO

PM/CYTO (Gpr3/)
Specimen
 
(Gpr3+/+)
 
(Gpr3/)
 
PM/CYTO (Gpr3+/+)
 
Follicle-enclosed
   oocytesd 3.1 ± 0.2
 (n = 22)a 7.1 ± 0.4
 (n = 30)b 2.3 ± 0.2c 
Isolated oocytese 8.8 ± 0.8
 (n = 19) 21.8 ± 1.2
 (n = 18) 2.5 ± 0.3c 
Isolated oocytes  (with optical
   correction)
 
4.1 ± 0.5
 (n = 19)a
 
10.1 ± 1.0
 (n = 18)b
 

 
a

These two values are not significantly different (t test, P = 0.08).

b

These two values are significantly different (t test, P = 0.01).

c

These two values are not significantly different (t test, P = 0.6).

d

Corresponds to Fig. 4 E.

e

Corresponds to Fig. 6 C.

Second and third columns indicate the ratios of plasma membrane GαsGFP fluorescence (PM) to cytoplasm GαsGFP fluorescence (CYTO) in the oocyte. Mean ± SEM (n = number of oocytes). Data for follicle-enclosed oocytes are from three mice of each genotype, and data for isolated oocytes are from two mice of each genotype. For each row, the last column indicates the value listed in the third column divided by the value listed in the second column. See the last section of Results and Fig. 7 for an explanation of the optical correction.

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