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Table I.

s immunofluorescence intensities for the plasma membrane and cytoplasm of Gpr3+/+and Gpr3/oocytes


Region of intensity measurement

Gpr3+/+

Gpr3−/−
Plasma membrane  intensity 91 ± 4 (n = 27)a 191 ± 13 (n = 15)a 
Cytoplasm intensity
 
29 ± 2 (n = 27)b
 
16 ± 2 (n = 15)b
 

Region of intensity measurement

Gpr3+/+

Gpr3−/−
Plasma membrane  intensity 91 ± 4 (n = 27)a 191 ± 13 (n = 15)a 
Cytoplasm intensity
 
29 ± 2 (n = 27)b
 
16 ± 2 (n = 15)b
 
a

These two values are significantly different (t test, P < 0.0001).

b

These two values are significantly different (t test, P = 0.0001).

s immunofluorescence intensities in the plasma membrane and cytoplasm of Gpr3+/+ and Gpr3−/− oocytes were measured as described in Fig. S1. Except for the detector gain, the confocal microscope settings were the same for all measurements; differences in gain were adjusted for by multiplying by constant conversion factors. Intensities are expressed as equivalent values at detector gain 800 on a scale of 0–255. Mean ± SEM (n = number of oocytes). Data are from two Gpr3+/+ and two Gpr3−/− mice. Ovaries from mice of the two genotypes were processed in parallel.

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