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Table I.

Microtubule dynamics parameters in basal membrane cytoplasts


Microtubule end

Parameter

Dynamic instability

New microtubules
Plus end Catastrophe frequency (min−10.95 ND 
 Rescue frequency (min−10.98 ND 
Plus end growth Rate (μm/min) 2.54 ± 0.65 (n = 34) 18.06 ± 3.26 (n = 10)a
 7.55 ± 1.24 (n = 17)a 
 Length (μm) 0.79 ± 0.37 6.92 ± 2.44 
 Duration (s) 17.3 ± 12.6 ND 
Plus end shortening Rate (μm/min) 5.06 ± 1.49 (n = 41) 12.73 ± 6.08 (n = 5) 
 Length (μm) 0.94 ± 0.36 ND 
 Duration (s) 13.2 ± 5.1 ND 
Minus end shortening
 
Rate (μm/min)
 
NA
 
7.49 ± 3.36 (n = 17)a
 

Microtubule end

Parameter

Dynamic instability

New microtubules
Plus end Catastrophe frequency (min−10.95 ND 
 Rescue frequency (min−10.98 ND 
Plus end growth Rate (μm/min) 2.54 ± 0.65 (n = 34) 18.06 ± 3.26 (n = 10)a
 7.55 ± 1.24 (n = 17)a 
 Length (μm) 0.79 ± 0.37 6.92 ± 2.44 
 Duration (s) 17.3 ± 12.6 ND 
Plus end shortening Rate (μm/min) 5.06 ± 1.49 (n = 41) 12.73 ± 6.08 (n = 5) 
 Length (μm) 0.94 ± 0.36 ND 
 Duration (s) 13.2 ± 5.1 ND 
Minus end shortening
 
Rate (μm/min)
 
NA
 
7.49 ± 3.36 (n = 17)a
 
a

See Fig. 5.

Values are means ± SD. NA, not applicable.

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