Average periodicity of IFT tracks
Cell . | Treatment . | Period (# starts/s) . | Number of reinforced flagella . | |
|---|---|---|---|---|
| lf3-2 (13c2) | Control | 4.3 ± 0.6 | 31 | |
| Colchicine | 4.1 ± 0.7 | 12 | ||
| LiCl | 4.0 ± 0.0 | 10 | ||
| 20 mM Na PPi | 4.1 ± 0.4 | 9 | ||
| ppi WO | 4.4 ± 0.7 | 16 | ||
| pf16 (13a2) | Control | 3.9 ± 0.8 | 24 | |
| 20 mM Na PPi | 4.1 ± 0.3 | 23 | ||
| ppi WO | 4.3 ± 0.6 | 8 | ||
| LiCl | 5.0 ± 0.4 | 4 | ||
| pf18 | Control | 3.9 ± 0.6 | 12 | |
| LiCl | 4.5 ± 0.6 | 11 | ||
| 20 mM Na PPi | 4.5 ± 0.7 | 11 | ||
| lf2 | Control | 4.3 ± 0.6 | 12 | |
| lf3 | Control | 3.8 ± 0.8 | 9 | |
| ulf2 | Control | 3.7 ± 0.6 | 7 | |
| AV | 4 ± 0.3 | 199 | ||
Cell . | Treatment . | Period (# starts/s) . | Number of reinforced flagella . | |
|---|---|---|---|---|
| lf3-2 (13c2) | Control | 4.3 ± 0.6 | 31 | |
| Colchicine | 4.1 ± 0.7 | 12 | ||
| LiCl | 4.0 ± 0.0 | 10 | ||
| 20 mM Na PPi | 4.1 ± 0.4 | 9 | ||
| ppi WO | 4.4 ± 0.7 | 16 | ||
| pf16 (13a2) | Control | 3.9 ± 0.8 | 24 | |
| 20 mM Na PPi | 4.1 ± 0.3 | 23 | ||
| ppi WO | 4.3 ± 0.6 | 8 | ||
| LiCl | 5.0 ± 0.4 | 4 | ||
| pf18 | Control | 3.9 ± 0.6 | 12 | |
| LiCl | 4.5 ± 0.6 | 11 | ||
| 20 mM Na PPi | 4.5 ± 0.7 | 11 | ||
| lf2 | Control | 4.3 ± 0.6 | 12 | |
| lf3 | Control | 3.8 ± 0.8 | 9 | |
| ulf2 | Control | 3.7 ± 0.6 | 7 | |
| AV | 4 ± 0.3 | 199 | ||
IFT track images were duplicated in Photoshop layers, and layers were shifted to reveal periodicity. Numerous spacings were tested and a minimum of four shifts was used to identify periodicity. Periodicity was only obtained on individual images at the spacings indicated.