Rate and frequency of anterograde and retrograde particle tracks
Cell . | . | Anterograde rate . | Retrograde rate . | Ratio A:R rates . | Average # anterograde (# counted) . | Average # retrograde (# counted) . | Ratio A:R . | Number of flagella scored . |
|---|---|---|---|---|---|---|---|---|
| . | . | μm/s . | μm/s . | . | Tracks/s . | Tracks/s . | Tracks/s . | . |
| lf3-2 (13c2) | Control | 1.9 ± 0.04 | 2.8 ± 0.04 | 0.68 | 1.4 (2104) | 1.5 (2185) | 0.93 | 37 |
| 20 mM Na PPi | 2.1 ± 0.04 | 2.6 ± 0.06 | 0.81 | 1.6 (583) | 1.6 (581) | 1.0 | 16 | |
| PPi washout | 2.2 ± 0.04 | 3.5 ± 0.03 | 0.63 | 1.8 (557) | 1.8 (558) | 1.0 | 16 | |
| 20 mM LiCl | 2.1 ± 0.03 | 3.5 ± 0.05 | 0.60 | 1.3 (403) | 1.5 (563) | 0.87 | 10 | |
| Colchicine | 1.9 ± 0.04 | 2.8 ± 0.06 | 0.68 | 1.5 (506) | 1.5 (521) | 1.0 | 13 | |
| pf18 | Control | 1.6 ± 0.04 | 2.5 ± 0.04 | 0.64 | 1.2 (722) | 1.4 (818) | 0.86 | 27 |
| 20 mM Na PPi | 1.8 ± 0.03 | 2.4 ± 0.04 | 0.75 | 1.2 (585) | 1.5 (705) | 0.80 | 13 | |
| 20 mM LiCl | 1.9 ± 0.02 | 2.8 ± 0.03 | 0.68 | 1.5 (731) | 1.7 (807) | 0.88 | 12 | |
| Hi Na+ low Ca2+ | 1.6 ± 0.04 | 2.0 ± 0.06 | 0.80 | 1.2 (407) | 1.0 (476) | 1.2 | 24 | |
| pf16 (13a2) | Control | 1.6 ± 0.04 | 2.6 ± 0.04 | 0.62 | 1.2 (1144) | 1.7 (1495) | 0.71 | 61 |
| 20 mM Na PPi | 2.0 ± 0.04 | 2.6 ± 0.04 | 0.77 | 1.7 (1571) | 1.8 (1675) | 0.94 | 27 | |
| PPi washout | 1.7 ± 0.03 | 2.5 ± 0.04 | 0.68 | 1.4 (447) | 1.7 (527) | 0.82 | 8 | |
| lf2 | Control | 1.8 ± 0.04 | 2.1 ± 0.06 | 0.86 | 0.9 (685) | 1.3 (829) | 0.69 | 22 |
| lf4 | 20 mM Na PPi | 1.9 ± 0.04 | 2.6 ± 0.6 | 0.73 | 1.3 (402) | 1.5 (486) | 0.87 | 8 |
| ulf2 | Control | 1.5 | 2.9 | 0.52 | 1.2 (402) | 1.4 (486) | 0.86 | 9 |
| Average for
all flagella | 1.9 | 2.7 | 0.70 | 1.4 (11,249) | 1.5 (12,712) | 0.93 | 316 |
Cell . | . | Anterograde rate . | Retrograde rate . | Ratio A:R rates . | Average # anterograde (# counted) . | Average # retrograde (# counted) . | Ratio A:R . | Number of flagella scored . |
|---|---|---|---|---|---|---|---|---|
| . | . | μm/s . | μm/s . | . | Tracks/s . | Tracks/s . | Tracks/s . | . |
| lf3-2 (13c2) | Control | 1.9 ± 0.04 | 2.8 ± 0.04 | 0.68 | 1.4 (2104) | 1.5 (2185) | 0.93 | 37 |
| 20 mM Na PPi | 2.1 ± 0.04 | 2.6 ± 0.06 | 0.81 | 1.6 (583) | 1.6 (581) | 1.0 | 16 | |
| PPi washout | 2.2 ± 0.04 | 3.5 ± 0.03 | 0.63 | 1.8 (557) | 1.8 (558) | 1.0 | 16 | |
| 20 mM LiCl | 2.1 ± 0.03 | 3.5 ± 0.05 | 0.60 | 1.3 (403) | 1.5 (563) | 0.87 | 10 | |
| Colchicine | 1.9 ± 0.04 | 2.8 ± 0.06 | 0.68 | 1.5 (506) | 1.5 (521) | 1.0 | 13 | |
| pf18 | Control | 1.6 ± 0.04 | 2.5 ± 0.04 | 0.64 | 1.2 (722) | 1.4 (818) | 0.86 | 27 |
| 20 mM Na PPi | 1.8 ± 0.03 | 2.4 ± 0.04 | 0.75 | 1.2 (585) | 1.5 (705) | 0.80 | 13 | |
| 20 mM LiCl | 1.9 ± 0.02 | 2.8 ± 0.03 | 0.68 | 1.5 (731) | 1.7 (807) | 0.88 | 12 | |
| Hi Na+ low Ca2+ | 1.6 ± 0.04 | 2.0 ± 0.06 | 0.80 | 1.2 (407) | 1.0 (476) | 1.2 | 24 | |
| pf16 (13a2) | Control | 1.6 ± 0.04 | 2.6 ± 0.04 | 0.62 | 1.2 (1144) | 1.7 (1495) | 0.71 | 61 |
| 20 mM Na PPi | 2.0 ± 0.04 | 2.6 ± 0.04 | 0.77 | 1.7 (1571) | 1.8 (1675) | 0.94 | 27 | |
| PPi washout | 1.7 ± 0.03 | 2.5 ± 0.04 | 0.68 | 1.4 (447) | 1.7 (527) | 0.82 | 8 | |
| lf2 | Control | 1.8 ± 0.04 | 2.1 ± 0.06 | 0.86 | 0.9 (685) | 1.3 (829) | 0.69 | 22 |
| lf4 | 20 mM Na PPi | 1.9 ± 0.04 | 2.6 ± 0.6 | 0.73 | 1.3 (402) | 1.5 (486) | 0.87 | 8 |
| ulf2 | Control | 1.5 | 2.9 | 0.52 | 1.2 (402) | 1.4 (486) | 0.86 | 9 |
| Average for
all flagella | 1.9 | 2.7 | 0.70 | 1.4 (11,249) | 1.5 (12,712) | 0.93 | 316 |
Anterograde and retrograde frequencies of IFT in flagella on control cells and cells treated with sodium pyrophosphate, colchicine, LiCl, or high Na+ low Ca2+.