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Table I.

Genetic interactions of Arp2 and Arp3 mutants


arp2-G302Y[WT] x

Double mutant phenotype

arp3-G302Y[ts, ss, fs] x

Double mutant phenotype
arp3-D11A  SL  arp2-D11A  SL 
arp3-T14A  SS (37°C)  arp2-T14A  SS (30°C) 
arp3-Q137A  nd  arp2-Q137A  NE 
arp3-Q137E  nd  arp2-Q137E  SS (25°C) 
arp3-D157E  SS (NaCl)  arp2-D157Ea  Sup (fs) 
arp3-V159N  NE  arp2-V159N  NE 
arp3-G302Y  SL  arp2-G302Y  SL 
arp3-F306A  NE  arp2-Y306Aa  Sup (ts,fs) 
las17-11  SL  las17-11  SL 
arp2-Y306A[WT]Double mutant phenotype arp3-F306A[WT]Double mutant phenotype 
arp3-D11A  Sup (ss, fs)  arp2-D11A  Sup (ss) 
arp2-G13Cb  Sup (ts, fs)  arp3-G15Cb  Sup (ss, fs) 
las17-11
 
 Sup (fs)
 
 las17-11
 
 SS (30°C)
 

arp2-G302Y[WT] x

Double mutant phenotype

arp3-G302Y[ts, ss, fs] x

Double mutant phenotype
arp3-D11A  SL  arp2-D11A  SL 
arp3-T14A  SS (37°C)  arp2-T14A  SS (30°C) 
arp3-Q137A  nd  arp2-Q137A  NE 
arp3-Q137E  nd  arp2-Q137E  SS (25°C) 
arp3-D157E  SS (NaCl)  arp2-D157Ea  Sup (fs) 
arp3-V159N  NE  arp2-V159N  NE 
arp3-G302Y  SL  arp2-G302Y  SL 
arp3-F306A  NE  arp2-Y306Aa  Sup (ts,fs) 
las17-11  SL  las17-11  SL 
arp2-Y306A[WT]Double mutant phenotype arp3-F306A[WT]Double mutant phenotype 
arp3-D11A  Sup (ss, fs)  arp2-D11A  Sup (ss) 
arp2-G13Cb  Sup (ts, fs)  arp3-G15Cb  Sup (ss, fs) 
las17-11
 
 Sup (fs)
 
 las17-11
 
 SS (30°C)
 
a

Suppression is of arp3-G302Y phenotype.

b

Intragenic interaction.

Genetic interactions were examined by dissecting at least seven tetrads for each cross and identifying double mutants using linked auxotrophic markers. The phenotypes of starting strains are shown in brackets. SL, synthetic lethal at 25°C; SS, synthetic sick; NE, no effect; Sup, suppression; ts, temperature sensitive; ss, salt sensitive; fs, formamide sensitive; nd, not determined. Growth under the condition shown in parentheses changes in the double mutant.

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