Skip to Main Content
Table I.

Concentration of the U4 snRNP in CBs is not changed upon expression of mutant SART3 (ΔCT10ΔRRM)


Expressed construct

Ratio of fluorescent intensities (CB/nuc.) ± SD (n)a
 LSm4 (U6)b
 
U4 snRNA
 
61K
 
None 1.82 ± 0.51 (124) 1.55 ± 0.27 (88) 1.71± 0.44 (70) 
SART3–GFP 1.58 ± 0.40 (130)
 (P = 0.0011)c 1.64 ± 0.47 (65)
 (P = 0.30)c 1.89 ± 0.53 (53)
 (P = 0.079)c 
ΔCT10ΔRRM–GFP
 
1.28 ± 0.27 (157)
 (P ≪ 10−10)c
 
1.79 ± 0.42 (112)
 (P = 0.0007)c
 
1.72± 0.49 (77)
 (P = 0.90)c
 

Expressed construct

Ratio of fluorescent intensities (CB/nuc.) ± SD (n)a
 LSm4 (U6)b
 
U4 snRNA
 
61K
 
None 1.82 ± 0.51 (124) 1.55 ± 0.27 (88) 1.71± 0.44 (70) 
SART3–GFP 1.58 ± 0.40 (130)
 (P = 0.0011)c 1.64 ± 0.47 (65)
 (P = 0.30)c 1.89 ± 0.53 (53)
 (P = 0.079)c 
ΔCT10ΔRRM–GFP
 
1.28 ± 0.27 (157)
 (P ≪ 10−10)c
 
1.79 ± 0.42 (112)
 (P = 0.0007)c
 
1.72± 0.49 (77)
 (P = 0.90)c
 
a

Ratio of average fluorescent intensity/pixel in CB and the nucleoplasm (nuc.); n = number of CBs evaluated.

b

Data taken from Stanek et al. (2003).

c

P values determined from the t test comparing fluorescent intensity ratios from the experimental data sets versus control ratios (shown in “None” row).

Close Modal

or Create an Account

Close Modal
Close Modal