Transcription factor activation following treatment of articular chondrocytes with BMP-7
Sample . | Smad4 . | SBE . | Pax-1 . |
|---|---|---|---|
| Control | 111.1 ± 1.2 | 2.4 ± 0.9 | 53.8 ± 0.7 |
| +BMP-7 | 481.2 ± 4.0 | 29.2 ± 0.4 | 54.4 ± 1.5 |
| +BMP-7/+S.H'ase | 217.7 ± 11 | 20.8 ± 1.4 | 53.6 ± 0.9 |
| Control | 34.6 ± 0.1 | - | - |
| S.H'ase | 32.0 ± 2.0 | - | - |
Sample . | Smad4 . | SBE . | Pax-1 . |
|---|---|---|---|
| Control | 111.1 ± 1.2 | 2.4 ± 0.9 | 53.8 ± 0.7 |
| +BMP-7 | 481.2 ± 4.0 | 29.2 ± 0.4 | 54.4 ± 1.5 |
| +BMP-7/+S.H'ase | 217.7 ± 11 | 20.8 ± 1.4 | 53.6 ± 0.9 |
| Control | 34.6 ± 0.1 | - | - |
| S.H'ase | 32.0 ± 2.0 | - | - |
Nuclei were isolated from control chondrocytes or after treatment with BMP-7, Streptomyces hyaluronidase alone (S.H'ase), or co-treatment with BMP-7 and S.H'ase. Transcription factor activation was determined as hybridization signal intensity and presented as the mean ± SEM.