Skip to Main Content
Table II.

Kinetic and thermodynamic parameters for the in vitro reaction between rxYFP and glutathione


pH

Kox(M)a

kox(M1min1)a

kred(M2min1)b
6.1 11.8 ± 0.9 25.5 ± 0.6 2.2 ± 0.2 
6.4 9.9 ± 0.3 32.6 ± 0.3 3.3 ± 0.1 
6.7 8.2 ± 0.5 41.6 ± 0.7 5.0 ± 0.3 
7.0c 6.8 ± 0.2 71.7 ± 0.9 10.6 ± 0.4 
7.3 7.2 ± 0.4 123.9 ± 0.5 17.2 ± 1.0 
7.6 7.6 ± 0.2 205.9 ± 1.0 27.0 ± 0.8 
7.9
 
6.8 ± 0.2
 
400.4 ± 4.4
 
59.0 ± 0.2
 

pH

Kox(M)a

kox(M1min1)a

kred(M2min1)b
6.1 11.8 ± 0.9 25.5 ± 0.6 2.2 ± 0.2 
6.4 9.9 ± 0.3 32.6 ± 0.3 3.3 ± 0.1 
6.7 8.2 ± 0.5 41.6 ± 0.7 5.0 ± 0.3 
7.0c 6.8 ± 0.2 71.7 ± 0.9 10.6 ± 0.4 
7.3 7.2 ± 0.4 123.9 ± 0.5 17.2 ± 1.0 
7.6 7.6 ± 0.2 205.9 ± 1.0 27.0 ± 0.8 
7.9
 
6.8 ± 0.2
 
400.4 ± 4.4
 
59.0 ± 0.2
 
a

Values are given as the mean ± SD (see Materials and methods).

b

Calculated as kox/Kox.

c

An E°′ value of −265 mV for rxYFP was calculated from the measured Kox at pH 7 and an E°′ value of −240 mV for the GSH/GSSG redox couple (Rost and Rapoport, 1964). This redox potential is in good agreement with that determined previously (Ostergaard et al., 2001).

Close Modal

or Create an Account

Close Modal
Close Modal