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Table II.

Immunocytochemical characterization of GAD-67+/EGFP+neurons in the perinatal and adult hippocampus



PV

DC

SOM

DIx

NeuN

P8
P40
P8
P40
P8
P40
P8
P40
P8
P40
DG           
GL 23 ± 4 52 ± 5 67 ± 7 71 ± 7 58 ± 8 94 ± 2 86 ± 2 70 ± 8 65 ± 8 
HL 14 ± 4 30 ± 4 9 ± 3 78 ± 5 68 ± 5 74 ± 6 92 ± 1 92 ± 2 86 ± 5 83 ± 7 
CA3           
SR 11 ± 3 45 ± 8 12 ± 3 76 ± 5 67 ± 6 66 ± 3 92 ± 4 83 ± 6 84 ± 6 83 ± 5 
SP 11 ± 2 53 ± 6 15 ± 3 67 ± 4 76 ± 2 72 ± 7 90 ± 4 86 ± 4 86 ± 2 92 ± 4 
CA1           
SR 28 ± 2 58 ± 5 95 ± 2 89 ± 1 90 ± 8 92 ± 8 
SP
 
0
 
69 ± 2
 
0
 
25 ± 2
 
62 ± 7
 
63 ± 2
 
95 ± 2
 
85 ± 2
 
88 ± 2
 
94 ± 6
 


PV

DC

SOM

DIx

NeuN

P8
P40
P8
P40
P8
P40
P8
P40
P8
P40
DG           
GL 23 ± 4 52 ± 5 67 ± 7 71 ± 7 58 ± 8 94 ± 2 86 ± 2 70 ± 8 65 ± 8 
HL 14 ± 4 30 ± 4 9 ± 3 78 ± 5 68 ± 5 74 ± 6 92 ± 1 92 ± 2 86 ± 5 83 ± 7 
CA3           
SR 11 ± 3 45 ± 8 12 ± 3 76 ± 5 67 ± 6 66 ± 3 92 ± 4 83 ± 6 84 ± 6 83 ± 5 
SP 11 ± 2 53 ± 6 15 ± 3 67 ± 4 76 ± 2 72 ± 7 90 ± 4 86 ± 4 86 ± 2 92 ± 4 
CA1           
SR 28 ± 2 58 ± 5 95 ± 2 89 ± 1 90 ± 8 92 ± 8 
SP
 
0
 
69 ± 2
 
0
 
25 ± 2
 
62 ± 7
 
63 ± 2
 
95 ± 2
 
85 ± 2
 
88 ± 2
 
94 ± 6
 

Sagittal sections from postnatal day 8 (P8) and 40 (P40) brains of CNP-EGFP transgenic mice were stained with different antibody combinations, as described in Materials and methods. Cells were analyzed in dentate gyrus (DG) granule layer (GL) and hylus (HL), and in CA3 and CA1 stratum radiatum (SR) and stratum pyramidale (SP). Percentages of GAD-67+/EGFP+ cells labeled with each marker are shown. Values represent mean ± SEM obtained from four different brains (six tissue sections per brain). Total cells counted were 1,080 for P8 and 770 for P40. PV, parvalbumin; DC, doublecortin; SOM, somatostatin.

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