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Table I.

Antigenic characterization of FACS®-purified perinatal and adult NG2+/EGFP+ cells




NG2

Ki67

LEX

DLX

EGFR

PSA

TUJ

GFAP
Postnatal (P2) 100 65.99 ± 0.42 26.0 ± 1.8 13.13 ± 2.2 97.52 ± 5.3 26.26 ± 0.30 10.17 ± 0.50 N/D 
Adult (P40)
 
100
 
26.43 ± 2.2
 
14.58 ± 1.8
 
9.62 ± 1.2
 
42.20 ± 3.4
 
16.23 ± 0.4
 
6.2 ± 1.0
 
N/D
 



NG2

Ki67

LEX

DLX

EGFR

PSA

TUJ

GFAP
Postnatal (P2) 100 65.99 ± 0.42 26.0 ± 1.8 13.13 ± 2.2 97.52 ± 5.3 26.26 ± 0.30 10.17 ± 0.50 N/D 
Adult (P40)
 
100
 
26.43 ± 2.2
 
14.58 ± 1.8
 
9.62 ± 1.2
 
42.20 ± 3.4
 
16.23 ± 0.4
 
6.2 ± 1.0
 
N/D
 

Postnatal day 2 (P2) and 40 (P40) brains from CNP-EGFP transgenic mice were dissociated and processed for NG2/EGFP FACS® purification, as described in Materials and methods. After FACS®, cells were plated on polyornithine-coated coverslips (20,000 cells per 35-mm coverslip) and processed for immunocytochemistry after 2 h. Percentages of NG2+/EGFP+ cells labeled with each marker are shown. Cells were not detected at P2 or P40 with the astrocyte marker GFAP (N/D). Values represent means ± SEM obtained from a total of five to six separate FACS® experiments and 10 microscopic fields per experiment. Total number of cells counted for each marker ranged between 2,787 and 3,251 at P2 and 2,020 and 2,485 at P40.

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