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Table I.

Identification of filaggrin as the protein absent in Matriptase/MT-SP1–deficient skin by mass spectrometry


Measured mass

Theoretical massa

Residue no.

Sequence
D
 
D
 

 

 
2461.4 2461.5 117–136 (R)GHQHQHQHQHEHEQPESGHR(Q) 
2197.1 2197.3 206–226 (R)QPSPSQSSDSQVHSGVQVEGR (R) 
2354.3 2353.5 206–227 (R)QPSPSQSSDSQVHSGVQVEGRR (G) 
2721.9 2721.5 202–226 (R)DRPRQPSPSQSSDSQVHSGVQVEGR (R) 
2632.6 2632.6 45–70 (R)GVSESQASDSEGHSDFSEGQAVGAHR (Q) 
2788.2
 
2788.8
 
44–70
 
(R)RGVSESQASDSEGHSDFSEGQAVGAHR(Q)
 

Measured mass

Theoretical massa

Residue no.

Sequence
D
 
D
 

 

 
2461.4 2461.5 117–136 (R)GHQHQHQHQHEHEQPESGHR(Q) 
2197.1 2197.3 206–226 (R)QPSPSQSSDSQVHSGVQVEGR (R) 
2354.3 2353.5 206–227 (R)QPSPSQSSDSQVHSGVQVEGRR (G) 
2721.9 2721.5 202–226 (R)DRPRQPSPSQSSDSQVHSGVQVEGR (R) 
2632.6 2632.6 45–70 (R)GVSESQASDSEGHSDFSEGQAVGAHR (Q) 
2788.2
 
2788.8
 
44–70
 
(R)RGVSESQASDSEGHSDFSEGQAVGAHR(Q)
 
a

Theoretical average masses were derived from the National Center for Biotechnology Information database identifying mouse filaggrin B (GenBank/EMBL/DDBJ accession no. B35026).

Skin lysates from newborn Matriptase/MT-SP1−/− mice and littermate controls were separated by SDS-PAGE, and proteins were stained with amido black. Two corresponding gel pieces, in the region where a major protein appeared to be absent in the Matriptase/MT-SP1−/− mice, were excised from parallel lanes containing skin lysates from Matriptase/MT-SP1−/− mice and control mice, and were subjected to in-gel trypsin digestion. The fragments listed represent peptides that were prominent in the control skin and were nondetectable in the Matriptase/MT-SP1−/− skin.

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