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Table I.

Effect of VASP on the motility behavior of beads functionalized with different surface densities of ActA




Surface
 density

Beads that form tails

Average time period before movement stops

molecules/100 nm2
%
min
− VASP 0.85 10 10–15 
+ VASP 0.85 97 60–90 
− VASP 1.36 90 40 
+ VASP 1.36 98       >120 
− VASP 1.85 94 45 
+ VASP 1.85 100       >120 
− VASP 2.12 97 60–80 
+ VASP
 
2.12
 
100
 
      >120
 



Surface
 density

Beads that form tails

Average time period before movement stops

molecules/100 nm2
%
min
− VASP 0.85 10 10–15 
+ VASP 0.85 97 60–90 
− VASP 1.36 90 40 
+ VASP 1.36 98       >120 
− VASP 1.85 94 45 
+ VASP 1.85 100       >120 
− VASP 2.12 97 60–80 
+ VASP
 
2.12
 
100
 
      >120
 

Movement of beads was recorded until the actin tails detached from the beads. The time at which 50% of the beads (n = 200) had lost their tails was noted.

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