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Table IV.

Percentage of beating EBs from Cripto^−/−ES cells transfected with Nodal antagonists

Construct	Protein	EBs scored	% of beating EBs
Empty vector	None	40	0
Empty vector	Cripto^{^a}	58	85
Cerberus	None	34	0
Cerberus	Cripto^{^a}	49	10.3
Cerberus-S	None	36	0
Cerberus-S	Cripto^{^a}	40	8.3

Construct	Protein	EBs scored	% of beating EBs
Empty vector	None	40	0
Empty vector	Cripto^{^a}	58	85
Cerberus	None	34	0
Cerberus	Cripto^{^a}	49	10.3
Cerberus-S	None	36	0
Cerberus-S	Cripto^{^a}	40	8.3

^a

2-d-old EBs were treated with 10 μg/ml of recombinant Cripto for 3 d.