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Table IV.

Percentage of beating EBs from Cripto/ES cells transfected with Nodal antagonists


Construct

Protein

EBs scored

% of beating EBs
Empty vector None 40 
Empty vector Criptoa 58 85 
Cerberus None 34 
Cerberus Criptoa 49 10.3 
Cerberus-S None 36 
Cerberus-S
 
Criptoa
 
40
 
8.3
 

Construct

Protein

EBs scored

% of beating EBs
Empty vector None 40 
Empty vector Criptoa 58 85 
Cerberus None 34 
Cerberus Criptoa 49 10.3 
Cerberus-S None 36 
Cerberus-S
 
Criptoa
 
40
 
8.3
 
a

2-d-old EBs were treated with 10 μg/ml of recombinant Cripto for 3 d.

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