Percentage of beating EBs from Cripto−/− ES cells transfected with either wt or Cripto mutant derivatives
Cells . | Construct . | EBs scored . | % of beating EBs . |
|---|---|---|---|
| DE7 | None | 97 | 0 |
| DE7 | Cripto wt | 56 | 98.2 |
| DE7 | N63I | 54 | 91.5 |
| DE7 | G71N | 54 | 0 |
| DE7 | T72A | 62 | 90.3 |
| DE7 | S77A | 60 | 95.0 |
| DE7 | F78A | 47 | 42.5 |
| DE7 | F78W | 60 | 95.0 |
| DE7 | H104A | 56 | 89.3 |
| DE7 | W107G | 57 | 7.6 |
| DE7 | R116G | 49 | 80.0 |
| DE7 | L122N | 103 | 92.0 |
| DE7 | Empty vector | 65 | 0 |
| DE14 | None | 85 | 0 |
| DE14 | Cripto wt | 54 | 94.4 |
| DE14 | G71N | 49 | 0 |
| DE14 | F78A | 45 | 66.0 |
| DE14 | W107G | 57 | 30.5 |
| DE14 | Empty vector | 71 | 0 |
Cells . | Construct . | EBs scored . | % of beating EBs . |
|---|---|---|---|
| DE7 | None | 97 | 0 |
| DE7 | Cripto wt | 56 | 98.2 |
| DE7 | N63I | 54 | 91.5 |
| DE7 | G71N | 54 | 0 |
| DE7 | T72A | 62 | 90.3 |
| DE7 | S77A | 60 | 95.0 |
| DE7 | F78A | 47 | 42.5 |
| DE7 | F78W | 60 | 95.0 |
| DE7 | H104A | 56 | 89.3 |
| DE7 | W107G | 57 | 7.6 |
| DE7 | R116G | 49 | 80.0 |
| DE7 | L122N | 103 | 92.0 |
| DE7 | Empty vector | 65 | 0 |
| DE14 | None | 85 | 0 |
| DE14 | Cripto wt | 54 | 94.4 |
| DE14 | G71N | 49 | 0 |
| DE14 | F78A | 45 | 66.0 |
| DE14 | W107G | 57 | 30.5 |
| DE14 | Empty vector | 71 | 0 |
Data are representative of at least two independent experiments. DE7 and DE14 are two independent Cripto−/− clones.