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Table III.

Percentage of beating EBs from Cripto/ ES cells transfected with either wt or Cripto mutant derivatives


Cells

Construct

EBs scored

% of beating EBs
DE7 None 97 
DE7 Cripto wt 56 98.2 
DE7 N63I 54 91.5 
DE7 G71N 54 
DE7 T72A 62 90.3 
DE7 S77A 60 95.0 
DE7 F78A 47 42.5 
DE7 F78W 60 95.0 
DE7 H104A 56 89.3 
DE7 W107G 57 7.6 
DE7 R116G 49 80.0 
DE7 L122N 103 92.0 
DE7 Empty vector 65 
DE14 None 85 
DE14 Cripto wt 54 94.4 
DE14 G71N 49 
DE14 F78A 45 66.0 
DE14 W107G 57 30.5 
DE14
 
Empty vector
 
71
 
0
 

Cells

Construct

EBs scored

% of beating EBs
DE7 None 97 
DE7 Cripto wt 56 98.2 
DE7 N63I 54 91.5 
DE7 G71N 54 
DE7 T72A 62 90.3 
DE7 S77A 60 95.0 
DE7 F78A 47 42.5 
DE7 F78W 60 95.0 
DE7 H104A 56 89.3 
DE7 W107G 57 7.6 
DE7 R116G 49 80.0 
DE7 L122N 103 92.0 
DE7 Empty vector 65 
DE14 None 85 
DE14 Cripto wt 54 94.4 
DE14 G71N 49 
DE14 F78A 45 66.0 
DE14 W107G 57 30.5 
DE14
 
Empty vector
 
71
 
0
 

Data are representative of at least two independent experiments. DE7 and DE14 are two independent Cripto−/− clones.

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