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Table I.

Percentage of beating EBs from Cripto/ ES cells transfected with either wt or ca form of human Alk4 or zebrafish Taram-A receptors


Cells

Construct

EBs scored

% of beating EBs
DE7 None 70 
DE7 Cripto wt 50 96.6 
DE7 Alk4 wt 76 
DE7 Alk4 ca 50 16.0 
DE7 Taram-A wt 55 
DE7 Taram-A ca 64 45.0 
DE7 Empty vector 56 
DE14 None 80 
DE14 Cripto wt 54 94.4 
DE14 Taram-A wt 50 1.9 
DE14 Taram-A ca 51 62.2 
DE14
 
Empty vector
 
60
 
0
 

Cells

Construct

EBs scored

% of beating EBs
DE7 None 70 
DE7 Cripto wt 50 96.6 
DE7 Alk4 wt 76 
DE7 Alk4 ca 50 16.0 
DE7 Taram-A wt 55 
DE7 Taram-A ca 64 45.0 
DE7 Empty vector 56 
DE14 None 80 
DE14 Cripto wt 54 94.4 
DE14 Taram-A wt 50 1.9 
DE14 Taram-A ca 51 62.2 
DE14
 
Empty vector
 
60
 
0
 

Data are representative of at least two independent experiments. DE7 and DE14 are two independent Cripto−/− clones.

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